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Calcium-enhanced exocytosis of gold nanoparticles
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10.1063/1.3486681
/content/aip/journal/apl/97/9/10.1063/1.3486681
http://aip.metastore.ingenta.com/content/aip/journal/apl/97/9/10.1063/1.3486681
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Figures

Image of FIG. 1.
FIG. 1.

(a) Experimental scheme showing labeling of a lipid bilayer using the lipophilic Laurdan dye. When excited with light at 340 nm, a redshift of the dye emission (illustrated by a change of dye emission color from dark to light) is induced when the bilayer undergoes a transition from the gel to the fluid phase. (b) Example of the redshift of the Laurdan dye emission, from a peak wavelength of 441–452 nm for a time lapse of 240 min. Excitation: 340 nm. (c) Confocal fluorescence image of HT-29 cells labeled with Laurdan, showing the cross-sections of the cell membranes as blue rings. Excitation: 340 nm.

Image of FIG. 2.
FIG. 2.

(a) GP values for cells incubated with different concentrations of AuNPs, measured immediately after the free nanoparticles were removed. The asterisks indicate statistically different GP values from that for the control samples (0 mg/mL AuNP concentration) . (b) Change in GP value over time for control cells (lower curve) and cells preincubated with AuNPs of 0.0005 mg/mL and treated with of 10 mM (upper curve).

Image of FIG. 3.
FIG. 3.

(a) Absorbance of the AuNPs collected from extracellular liquids vs concentration, read at 490 nm. Exposure time of cells to : 4 h. Original AuNP concentration: 0.5 mg/mL. Incubation time of the AuNPs with the cells: 2 h. (b) GP values of cells treated (light-colored bars) and untreated with AuNPs (dark-colored bars) in culture medium with different concentrations. Incubation time of the AuNPs with the cells: 4 h. AuNP concentration: 0.005 mg/mL. The asterisks indicate statistically different absorbance/GP values from that for the control samples (0 mM concentration) .

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/content/aip/journal/apl/97/9/10.1063/1.3486681
2010-09-03
2014-04-16
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Calcium-enhanced exocytosis of gold nanoparticles
http://aip.metastore.ingenta.com/content/aip/journal/apl/97/9/10.1063/1.3486681
10.1063/1.3486681
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