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Highly-efficient single-cell capture in microfluidic array chips using differential hydrodynamic guiding structures
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    Jaehoon Chung1,a), Young-Ji Kim1,b) and Euisik Yoon1,2,c)
    + View Affiliations - Hide Affiliations
    1 Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, Michigan 48109, USA
    2 Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA
    a) Present address: Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts 02114, USA.
    b) Present address: OLED Technology Center, Samsung Mobile Display, Yongin 446-811, South Korea.
    c) Author to whom correspondence should be addressed. Present address: 1301 Beal Ave, Ann Arbor MI 48109, USA. Tel.: 734-615-4469. FAX: 734-763-9324. Electronic mail: esyoon@umich.edu.
    Appl. Phys. Lett. 98, 123701 (2011); http://dx.doi.org/10.1063/1.3565236


Image of FIG. 1.
FIG. 1.

(a) Schematic diagram of a unit microwell with hydrodynamic guiding structures for a high capturing efficiency, (b) illustration of fabrication procedures, (c) photograph of the fabricated microwell array chip and the magnified view of four microwells, (d) four microwell structures with various channel length ratios of path A and path B.

Image of FIG. 2.
FIG. 2.

(a) Estimated capturing efficiencies as a function of channel width and channel length ratios and (b) COMSOL simulation results of flow velocity distribution and streamlines. (The inserted bar indicates velocity profiles at an injecting flow speed of 1 mm/s and the white lines are velocity streamlines).

Image of FIG. 3.
FIG. 3.

(a) Capturing efficiency vs length ratio when a channel width ratio is 2.5 (error bar represents its standard deviation, ). (b) Photograph of captured single green-fluorescence-activated PC3 cells in microwell array. Only one PC3 cell was captured at each capturing site. In the image, 39 microwells are occupied with single cells out of 42 microwells, (d) and (e) Single cell culture for three days: photographs were taken in an identical microwell at day 0 (c) and day 3 (d).


Generic image for table
Table I.

Dimension of path A and path B.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Highly-efficient single-cell capture in microfluidic array chips using differential hydrodynamic guiding structures