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Real-time monitoring of mitochondrial adenosine -triphosphate synthesis and hydrolysis by surface infrared spectroscopy
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10.1063/1.3571551
/content/aip/journal/apl/98/13/10.1063/1.3571551
http://aip.metastore.ingenta.com/content/aip/journal/apl/98/13/10.1063/1.3571551
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Figures

Image of FIG. 1.
FIG. 1.

(a) A schematic of the solution cells. A Si prism for MIR-IRAS measurements and an oxygen electrode are attached to the sides of the solution cell. Volume of the sample solution: 5.2 ml. (b) A cross section of the solution cells. A magnetic bar is continuously stirred by a mini magnetic stirrer attached to the bottom of the solution cell during the MIR-IRAS measurement.

Image of FIG. 2.
FIG. 2.

(a) IR spectral changes in mitochondrial suspension after addition of ADP. (b) IR spectral changes in ADP-treated mitochondrial suspension after addition of SF6847. For (a) and (b), the reference was the spectrum collected just before addition of ADP. (c) IR spectra of (i) 25 mM ATP and (ii) 25 mM ADP dissolved in phosphate buffer (200 mM sucrose, 20 mM KCl, 3 mM , and 100 mM potassium phosphate, ). The reference was the IR spectrum of the phosphate buffer. Curve (iii) is the sum spectrum of (i) and (ii) when the mixing ratio (ATP:ADP) is 1:1.

Image of FIG. 3.
FIG. 3.

(a) (i) Difference spectrum between the bottom (36 min) and top (3 min) curves of Fig. 2(a). (ii) Spectral change in Pi-buffer containing ADP during incubation for . (iii) Spectral change in mitochondrial suspension induced by addition of Pi-buffer. [(iv) and (v)] Difference spectrum of ADP-treated mitochondrial suspension before and after addition of (iv) SF6847 or (v) ethanol. (vi) Difference spectrum of mitochondrial suspension before and after addition of SF6847 in the absence of ADP. (b) Typical time courses of the peak intensity at obtained from one experimental pair of (●) , (○) , and (△) . In this graph, “0 min” means when the IRAS measurement just after the addition of ADP was finished (3 min after the ADP addition). The arrow indicates when SF6847 was added to the suspension (33 min). In the case of SF6847(–), ethanol (solvent of SF6847) was added to the mitochondrial suspension.

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/content/aip/journal/apl/98/13/10.1063/1.3571551
2011-03-29
2014-04-23
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Real-time monitoring of mitochondrial adenosine 5′-triphosphate synthesis and hydrolysis by surface infrared spectroscopy
http://aip.metastore.ingenta.com/content/aip/journal/apl/98/13/10.1063/1.3571551
10.1063/1.3571551
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