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Excitation of Cy5 in self-assembled lipid bilayers using optical microresonators
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View: Figures


Image of FIG. 1.
FIG. 1.

Optical resonant cavity excitation of Cy5-conjugated lipids. (a) A rendering of a spherical resonant microcavity highlighting the location of the optical field. (b) The optical field intensity inside (blue line) and outside (red line) of the cavity with a 5 nm bilayer at 980 nm. The decay length is approximately 100 nm. The n, l, and m represent the radial, polar, and azimuthal mode number, respectively. (c) An optical micrograph of the silica microsphere microcavity before lipid bilayer attachment. (d) A widefield fluorescence micrograph of the microcavity after lipid bilayer attachment. (e) A close-up fluorescence micrograph of the microcavity surface in buffer. (f) The same microcavity after the addition of a fluorescence quenching agent.

Image of FIG. 2.
FIG. 2.

The measurements of the photon lifetime or Q factor, which indicates the interaction strength between the whispering gallery mode and the lipid bilayer. The quality factor was determined from the full-width at half maximum (FWHM, ) of the Lorentzian fit (dashed red line) to the experimental data (solid black line). This measurement was performed using an undoped lipid bilayer at (a) 633 nm and (b) 980 nm, and using a Cy5 conjugated lipid bilayer at (c) 633 nm and (d) 980 nm.

Image of FIG. 3.
FIG. 3.

The fluorescent emission spectra of the Cy5 in the lipid bilayer excited using the microcavity. (a) Schematic of test and measurement setup. The evanescent field of the microcavity excites the Cy5-conjugated lipids, and the emission is detected by the spectrograph. The optical power is fiber coupled into and out of the resonant cavity, and the collected fluorescence signal is fiber coupled into the spectrograph. (b) The emission from the Cy5-conjugated lipid bilayer which is excited by the optical microresonator. The peak maximum is located at 670.11 nm. The 633 nm excitation laser is blocked using a filter.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Excitation of Cy5 in self-assembled lipid bilayers using optical microresonators