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A schematic diagram of Optofluidic Tomography setup is shown. (Not to scale) The sample to be imaged flows within a microfluidic channel with distance to the active area of the digital sensor array, and is illuminated from multiple angles by partially coherent light emanating from a large aperture (e.g., 0.05–0.1 mm) placed away from the sample.
(Top) Lensfree raw holograms of a C. elegans sample at three different illumination angles (, 34°, and −34°) are presented. (Bottom) SR holograms for the same illumination angles are shown. As expected, lensfree holograms are wider for the tilted illumination angles when compared to the vertical illumination case.
Reconstruction results of tilted angle illumination in holographic optofluidic microscopy are shown, in both amplitude and phase, along with a 40× objective-lens microscope comparison image corresponding to the vertical cross-section of the worm. Raw and SR holograms of the same sample are illustrated in Fig. 2.
Tomographic optofluidic imaging of C. elegans is illustrated at different depth slices ( to ). The FWHM of the amplitude of the line profile of the worm along direction is , which roughly agrees with the thickness of its body. The colorbar applies only to the tomographic images. Scale bars: . For the other depth slices, the readers can refer to the Supplementary Movie (enhanced online). [URL: http://dx.doi.org/10.1063/1.3548564.1]10.1063/1.3548564.1
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