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Microfluidic resonant waveguide grating biosensor system for whole cell sensing
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1.
1.Y. Fang, G. Li, and J. Peng, FEBS Lett. 579, 6365 (2005).
http://dx.doi.org/10.1016/j.febslet.2005.10.019
2.
2.Y. Fang and A. M. Ferrie, FEBS Lett. 582, 558 (2008).
http://dx.doi.org/10.1016/j.febslet.2008.01.021
3.
3.T. Kenakin, Nat. Rev. Drug Discovery 8, 617 (2009).
http://dx.doi.org/10.1038/nrd2838
4.
4.A. Kebig, E. Kostenis, K. Mohr, and M. Mohr-Andra, J. Recept. Signal Transduct. Res. 29, 140 (2009).
http://dx.doi.org/10.1080/10799890903047437
5.
5.Y. Fang, Drug Discov. Today. Technol. 7, e5 (2010).
http://dx.doi.org/10.1016/j.ddtec.2010.05.001
6.
6.C. W. Scott and M. F. Peters, Drug Discov. Today 15, 704 (2010).
http://dx.doi.org/10.1016/j.drudis.2010.06.008
7.
7.Y. Fang, Expert Opin. Drug Discov. 5, 1237 (2010).
http://dx.doi.org/10.1517/17460441.2010.533652
8.
8.Y. Fang, A. M. Ferrie, N. H. Fontaine, J. Mauro, and J. Balakrishnan, Biophys. J. 91, 1925 (2006).
http://dx.doi.org/10.1529/biophysj.105.077818
9.
9.Y. Fang, Sensors 7, 2316 (2007).
http://dx.doi.org/10.3390/s7102316
10.
10.V. Goral, Q. Wu, H. Sun, and Y. Fang, FEBS Lett. 585, 1054 (2011).
http://dx.doi.org/10.1016/j.febslet.2011.03.003
11.
11.R. Bergman, W. J. Miller, M. L. Morrell, T. M. Roswech, and P. K. Yuen, U.S. Patent 7824624.
12.
12.A. M. Ferrie, Q. Wu, and Y. Fang, Appl. Phys. Lett. 97, 223704 (2010).
http://dx.doi.org/10.1063/1.3522894
13.
13.A. J. Morris and C. C. Malbon, Physiol. Rev. 79, 1373 (1999).
14.
14.Y. Fang, G. Li, and A. M. Ferrie, J. Pharmacol. Toxicol. Methods 55, 314 (2007).
http://dx.doi.org/10.1016/j.vascn.2006.11.001
15.
15.W. M. Deen, Analysis of Transport Phenomena (Oxford University Press, New York, 1998).
16.
16.S. Chien, Am. J. Physiol. Heart Circ. Physiol. 292, H1209 (2007).
http://dx.doi.org/10.1152/ajpheart.01047.2006
17.
17.M. J. Lohse, J. L. Benovic, M. G. Caron, and R. J. Lefkowitz, J. Biol. Chem. 265, 3202 (1990).
18.
18.S. Rajagopal, K. Rajagopal, and R. J. Lefkowitz, Nat. Rev. Drug Discovery 9, 373 (2010).
http://dx.doi.org/10.1038/nrd3024
19.
19.E. Tran and Y. Fang, J. Recept. Signal Transduct. Res. 29, 154 (2009).
http://dx.doi.org/10.1080/10799890903052544
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FIG. 1.

A microfluidic RWG biosensor system for whole cell sensing. (a) A photo of a prototype system. (b) A bottom view photo of a biosensor plate containing a functional biosensor array. A colored buffer solution was introduced to illustrate the microchamber geometry. (c) The geometry of the T-shaped microchamber. (d) A schematic of side by side perfusion scheme.

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FIG. 2.

The DMR of confluent A431 cells upon perfusion with epinephrine and forskolin under different conditions. (a) The DMR within the detection area in comparison with that within the referencing area, wherein 5 nM epinephrine (EPI) and the buffer solutions were perfused side by side, respectively. (b) The DMR of cells responding to perfusion with different solutions: 5 nM EPI in the absence and presence of propranolol, and forskolin (FSK) in the absence and presence of propranolol. Perfusion rate: . The standard deviation represents 3 replicates in measurements. Schematic was the perfusion schemes.

Image of FIG. 3.

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FIG. 3.

The desensitization and resensitization of A431 cells. (a) The sustainability of the EPI DMR. The cells were first perfused with 5 nM EPI (the first perfusion). 50 min later the cells were perfused with the buffer, or 5 nM EPI for another 50 min (the second perfusion). The repeated stimulation with the buffer was used as a negative control. (b) The resensitization of A431 cells to repeated perfusions. An additional perfusion with the buffer (25 min) was introduced between the first (5 nM epinephrine, or forskolin) and third perfusions with a respective agonist ( forskolin, or 5 nM epinephrine, respectively). A short pause to reload different sample solutions occurred before the third perfusion. All perfusion rates were . The standard deviation represents three replicates in measurements. Schematic was the perfusion schemes.

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/content/aip/journal/apl/98/16/10.1063/1.3582611
2011-04-19
2014-04-16

Abstract

We report on a fluidic resonant waveguide grating (RWG) biosensorsystem that enables medium throughput measurements of cellular responses under microfluidics in a 32-well format. Dynamic mass redistribution assays under microfluidics differentiate the cross-desensitization process between the -adrenoceptor agonist epinephrine and the adenylate cyclase activator forskolin mediated signaling. This system opens new possibility to study cellular processes that are otherwise difficult to achieve using conventional RWG configurations.

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Scitation: Microfluidic resonant waveguide grating biosensor system for whole cell sensing
http://aip.metastore.ingenta.com/content/aip/journal/apl/98/16/10.1063/1.3582611
10.1063/1.3582611
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