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Schematic of a FOPO-based multimodal microscope. PBS is a polarization beam splitter cube. M1 is a 1064 nm short pass mirror. M2 is a 1064 nm long pass dichroic mirror. M3 is a protected silver cavity end mirror. M4 is a 940 nm short pass dichroic mirror. PP represents a prism pair. F1-F3 are bandpass filters. SB stands for a laser scanning box. PMT1 and PMT2 are photomultipliers. The inset shows energy diagrams for CARS and TPEF modalities.
FOPO output performance. (a) Tuning curves for ZDW950 and (b) ZDW1030. (c) Measured FROG spectrogram and (d) retrieved FROG spectrogram. (e) Reconstructed amplitude (solid) and phase (dashed) for the FOPO implemented with ZDW1030.
Imaging deuterated lipid droplets in 3T3-L1 cells with FOPO-based CARS. (a) Transmission image. (b) Forward detected CARS image at . (c) Raman spectrum obtained at the point indicated by the arrow in (a). C-D stretch is indicated by asterisk. The average pump and Stokes powers were 4 mW and 25 mW at the sample position, respectively. The total integration time per pixel was .
Multimodal CARS/TPEF imaging of rat spinal cord. (a) Forward detected CARS image of myelin sheath at . (b) TPEF image of axons labeled with NF160/alex488 excited using the pump at 1032 nm. (c) Overlaid CARS and TPEF images. (d) Magnified segment of the overlaid images. The average pump and Stokes powers were 3.3 mW and 15 mW at the sample position, respectively.
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