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Volume 98, Issue 24, 13 June 2011
Nanowires and nanotubes can be examined in the transmission electron microscope under an applied bias. Here we introduce a model-independent method, which allows the charge distribution along a nanowire or nanotube to be measured directly from the Laplacian of an electron holographic phase image. We present results from a biased bundle of carbon nanotubes, in which we show that the charge density increases linearly with distance from its base, reaching a value of near its tip.
- BIOPHYSICS AND BIO-INSPIRED SYSTEMS
98(2011); http://dx.doi.org/10.1063/1.3599586View Description Hide Description
In this letter we describe and fabricate an optimized version of micromirrors for three-dimensional (3D) single-particle tracking in living cells. Our technology is based on parallel arrays of single facet mirrors, which are introduced into the field of view of an optical microscope to provide one tilted view projected alongside the direct fluorescent sample image. Our method outperforms previously published V-shaped micromirrors technologies, as demonstrated by a ten-fold enhanced 3D positioning accuracy and a 1.7-fold reduced bleaching rate. We envision that our technology will be particularly useful for high-throughput 3D live cell imaging.