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Forward light scattering for highly sensitive detection of aggregation in crystallizing protein solutions
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10.1063/1.3603932
/content/aip/journal/apl/98/26/10.1063/1.3603932
http://aip.metastore.ingenta.com/content/aip/journal/apl/98/26/10.1063/1.3603932
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

(Color online) Schematic of the liquid sample cell and the forward light scattering measurement.

Image of FIG. 2.
FIG. 2.

(Color online) Radiation patterns of SLS. (a) SLS pattern for the small silica particles dispersed in water [0.05 wt. % (8.33 mM)]; (b) lysozyme crystallization solution with the precipitant NaCl [20 mg/ml (1.40 mM) HEWL and 3.0% (w/v) (0.512 M) NaCl]. Insets: polar coordinate displays.

Image of FIG. 3.
FIG. 3.

(Color online) Scattered light intensity as a function of scattering angle, for the HEWL solutions with 0% NaCl (black curve), 1.5% (w/v) NaCl (blue curve) and 3.0% (w/v) NaCl (red curve).

Image of FIG. 4.
FIG. 4.

(Color online) Dependence of the averaged scattered light intensity on the NaCl concentration. The squares (black) and the closed circles (red) represent HEWL concentrations of 20 and 30 mg/ml, respectively.

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/content/aip/journal/apl/98/26/10.1063/1.3603932
2011-06-28
2014-04-20
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Forward light scattering for highly sensitive detection of aggregation in crystallizing protein solutions
http://aip.metastore.ingenta.com/content/aip/journal/apl/98/26/10.1063/1.3603932
10.1063/1.3603932
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