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Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles
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10.1063/1.3554415
/content/aip/journal/apl/98/9/10.1063/1.3554415
http://aip.metastore.ingenta.com/content/aip/journal/apl/98/9/10.1063/1.3554415

Figures

Image of FIG. 1.
FIG. 1.

Formation of residual gas bubbles and cellular displacements by nanoparticle LIB. [(a) and (d)] Residual gas bubbles formed in a circle over after the LIB of 500 nm [video 1] and [video 3] nanoparticles. [(b) and (e)] Nanoparticle LIB in the presence of CHO-K1 cells indicating cellular displacements over after the LIB of 500 nm [video 2] and [video 4] nanoparticles. [(c) and (f)] Cell morphology, 5 s after the LIB of 500 nm and nanoparticles (enhanced online). [URL: http://dx.doi.org/10.1063/1.3554415.1] [URL: http://dx.doi.org/10.1063/1.3554415.3] [URL: http://dx.doi.org/10.1063/1.3554415.2] [URL: http://dx.doi.org/10.1063/1.3554415.4]10.1063/1.3554415.110.1063/1.3554415.310.1063/1.3554415.210.1063/1.3554415.4

Image of FIG. 2.
FIG. 2.

Optimization of transfection of Mito-DsRed encoding plasmids into multiples of CHO-K1 cells by LIB parameters. (a) Number of cells transfected with different nanoparticle sizes at the fixed axial location of . (b) Number of cells transfected at different axial locations with the fixed particle size of 500 nm. The error bars indicate .

Image of FIG. 3.
FIG. 3.

Simultaneous multisite targeted nanosurgery of cells using SLMs [video 5]. (a) Three trapped 500 nm nanoparticles placed on an equilateral triangle with side of positioned at above the cell monolayer before LIB. (b) Cell morphology, 5 s after the simultaneous LIB of the three trapped nanoparticles (enhanced online). [URL: http://dx.doi.org/10.1063/1.3554415.5]10.1063/1.3554415.5

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/content/aip/journal/apl/98/9/10.1063/1.3554415
2011-03-02
2014-04-19
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles
http://aip.metastore.ingenta.com/content/aip/journal/apl/98/9/10.1063/1.3554415
10.1063/1.3554415
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