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(Color online) Schematic drawing of the experimental apparatus. Plasma reactor has 2 nozzles ejecting plasma with dimension of 20 mm× 1 mm.
(Color online) (a) Changes in scar appearance over 6 weeks in the plasma and control groups. The additional wound seen in week 4 in the plasma group is a scratch from another mouse. (b) Hematoxylin and eosin-stained scar tissue from each group. The control group showed slightly increased fibrosis compared with the plasma group.
(Color online) Comparison of the results (a) comparison of the ratio of scar/normal areas of the LDPI value in the 2 groups. In the early stages of the test, the control group exhibited a somewhat increased ratio, indicating that scar generation was active. (b) Relative reduction in scar area over the course of treatment. The plasma group showed a continuous reduction in scar area until the end of the test period. In contrast, the control group tended to exhibit a persistent scar without further reduction in the scar area.
(a) Plasma induces cell death of HTSF. Cells were pretreated with or without NAC (10 mM) for 30 min before plasma treatment. After 24 h, cells were stained with FITC-Annexin V and then checked cell death rates. Measurement was obtained as relative fluorescence units (RFU) normalized to Staurosporine-treated scar cells and the results shown represent the mean of triplicates of three separate experiments ±SD, p < 0.01. (b) Plasma stimulates ROS formation in HTSF cells. Cells were treated with or without plasma and then immediately ROS generation was determined for 15 min, using a chemiluminescence assay. The average ±SD of three experiments is shown.
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