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Probing the physiology of ASH neuron in Caenorhabditis elegans using electric current stimulation
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10.1063/1.3615821
/content/aip/journal/apl/99/5/10.1063/1.3615821
http://aip.metastore.ingenta.com/content/aip/journal/apl/99/5/10.1063/1.3615821
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Figures

Image of FIG. 1.
FIG. 1.

(Color online) (a) The e-chip for electrically stimulating single worms. It consists of a worm trap, a set of ITO electrodes (labeled as 1, 2, and 3), an array of PDMS micropillars, (labeled as 4) and a flush channel (labeled as 5). Scale bar, 150 μm. Magnified views and the step architecture of the e-chip are shown on the right. Scale bars, 100 μm. (b) Pseudocolor-enhanced FRET (fluorescence resonance energy transfer) image of a trapped worm (marked by the dashed square in (a)). The ASH neuron is highlighted with the arrow in the two FRET channels (CFP and YFP). Scale bar, 5 μm.

Image of FIG. 2.
FIG. 2.

(Color online) Age-dependent effects of electric current of positive polarity in ASH. (a) Individual curves represent an average of 15 recordings from Day 1, Day 3, and Day 5 worms. Shaded regions represent standard error of mean. The dashed line represents the presence of the stimulus. (b) Mean values of the peak, α, and β constants of the calcium transients for three different ages. Error bars represent standard error of mean.

Image of FIG. 3.
FIG. 3.

(Color online) Effect of electric current of negative polarity in ASH in Day 1 worms. Individual curves represent an average of 15 recordings corresponding to electric current magnitudes of 0.001 μA, 0.01 μA, and 0.1 μA, respectively. ASH is hyperpolarized when stimulated with a current of 0.1 μA. Shaded regions represent standard error of mean.

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/content/aip/journal/apl/99/5/10.1063/1.3615821
2011-08-01
2014-04-21
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Probing the physiology of ASH neuron in Caenorhabditis elegans using electric current stimulation
http://aip.metastore.ingenta.com/content/aip/journal/apl/99/5/10.1063/1.3615821
10.1063/1.3615821
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