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(Color online) (a) The e-chip for electrically stimulating single worms. It consists of a worm trap, a set of ITO electrodes (labeled as 1, 2, and 3), an array of PDMS micropillars, (labeled as 4) and a flush channel (labeled as 5). Scale bar, 150 μm. Magnified views and the step architecture of the e-chip are shown on the right. Scale bars, 100 μm. (b) Pseudocolor-enhanced FRET (fluorescence resonance energy transfer) image of a trapped worm (marked by the dashed square in (a)). The ASH neuron is highlighted with the arrow in the two FRET channels (CFP and YFP). Scale bar, 5 μm.
(Color online) Age-dependent effects of electric current of positive polarity in ASH. (a) Individual curves represent an average of 15 recordings from Day 1, Day 3, and Day 5 worms. Shaded regions represent standard error of mean. The dashed line represents the presence of the stimulus. (b) Mean values of the peak, α, and β constants of the calcium transients for three different ages. Error bars represent standard error of mean.
(Color online) Effect of electric current of negative polarity in ASH in Day 1 worms. Individual curves represent an average of 15 recordings corresponding to electric current magnitudes of 0.001 μA, 0.01 μA, and 0.1 μA, respectively. ASH is hyperpolarized when stimulated with a current of 0.1 μA. Shaded regions represent standard error of mean.
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