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A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging
D. Débarre, W. Supatto, A.-M. Pena, A. Fabre, T. Tordjmann, L. Combettes, M.-C. Schanne-Klein, and E. Beaurepaire, Nat. Methods 3, 47–53 (2006).
V. A. Orlovich, Y. I. Malakhov, Y. M. Popov, D. N. Busko, M. B. Danailov, A. A. Demidovich, P. A. Apanasevich, and R. V. Chulkov, Laser Phys. Lett. 9(11), 770 (2012).
For example Spectraphysics “InSight DS” or Coherents “Chameleon Discovery.”
Femtosecond Optical Parametric Amplifier “Bodega,” Calmar Lasers, Palo Alto, CA, USA.
We demonstrate a femtosecond single pass Raman generator based on an YVO4 crystal pumped by a high energy fiber laser at a wavelength of 1064 nm and a repetition rate of 1 MHz. The Raman generator shifts the pump wavelength to 1175 nm, in a broadband spectrum, making it suitable for multi-photon microscopy. We use the Raman generator for third harmonic generation imaging of live plant specimens as well as for two-photon
imaging of red fluorescent protein expressing HeLa cells. We demonstrate that the photo-damage to a live specimen is low.
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