No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.
Microfluidic chip-based synthesis of alginate microspheres for encapsulation of immortalized human cells
1.X. Wang, W. Wang, J. Ma, X. Guo, X. Yu, and X. Ma, Biotechnol. Prog. 22, 791 (2006).
4.Z. H. Hu, S. Z. Chen, Y. Jin, Y. Xiong, W. Wang, X. J. Ma, and M. Song, Singapore Med. J. 47, 504 (2006).
5.R. Calafiore, G. Basta, G. Luca, M. Calvitti, G. Calabrese, L. Racanicchi, G. Macchiarulo, F. Mancuso, L. Guido, and P. Brunetti, Biotechnol. Appl. Biochem. 39, 159 (2004).
7.The prevention of diabetes and its complications, WHO Review 2006.
8.C. M. Shi and T. M. Cheng, World J. Gastroenterol. 10, 2550 (2004).
9.R. Calafiore, G. Basta, G. Luca, A. Lemmi, M. P. Montanucci, G. Calabrese, L. Racanicchi, F. Mancuso, and P. Brunetti, Diabetes Care 29, 137 (2006).
10.J. C. Ogbonna, M. Matsumura, and H. Kataoka, Process Biochem. (Oxford, U.K.) 26, 109 (1991).
14.D. Poncelet, R. Lencki, C. Beaulieu, J. P. Halle, and R. J. Neufeld, Appl. Microbiol. Biotechnol. 38, 39 (1992).
16.Y. Gao, H. C. Jiang, J. Xu, S. H. Pan, and Y. D. Li, Transplant. Proc. 37, 4589 (2005).
20.G. L. Fiszman, A. L. Karara, L. M. E. Finocchiaro, and G. C. Glikin, Electronic Journal of Biotechnology (2002), available at: http://www.ejbiotechnology.info/content/vol5/issue3/full/5/
Article metrics loading...
Cellular transplantation is a promising technology with great clinical potential in regenerative medicine and disease management. However, effective control over patient immunological response is essential. The encapsulation of cells within hydrogel microspheres is an increasingly prevalent method for the protection of cellular grafts from immune rejection. Microfluidic “chip” reactors present elegant solutions to several capsule generation issues, including the requirement for intercapsule uniformity, high reproducibility, and sterile, good manufacturing practice compliance. This study presents a novel method for the on-chip production of stable, highly monodisperse alginate microspheres and demonstrates its utility in the encapsulation of an immortalized human-derived cell line. Four populations of immortalized human embryonic kidney cells (HEK293) were encapsulated on chip within monodisperse alginate capsules. Cell viability measurements were recorded for each of the four encapsulated populations for .
Full text loading...
Most read this month