No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.
Development and evaluation of realistic microbioassays in freely suspended droplets on a chip
1.C. L. Baylis, in Detecting Pathogens in Food, edited by T. A. McMeekin (CRC LLC, Boca Raton, FL, 2004), p. 217.
2.L. B. Bangs, Pure Appl. Chem. 68, 1873 (1996).
3.P. E. Andreotti, G. V. Ludwig, A. H. Peruski, J. J. Tuite, S. S. Morse, and L. F. Peruski, Jr., BioTechniques 35, 850 (2003).
5.J. A. Molina-Bolivar, and F. Galisteo-Gonzalez, J. Macromol. Sci., Polym. Rev.C45, 59 (2005).
6.L. B. Bangs and M. T. Kenny, Ind. Res. 18, 46 (1976).
7.G. E. M. Tovar and A. Weber, Dekker Encyclopedia of Nanoscience and Nanotechnology, edited by J. A. Schwarz, C. I. Contescu, and K. Putyera (Marcel Dekker, New York, 2004), p. 277.
8.C. R. Lowe, B. F. Y. Y. Hin, D. C. Cullen, S. E. Evans, L. D. G. Stephens, and P. Maynard, J. Chromatogr. 510, 347 (1990).
10.L. B. Bangs, J. Clin. Immunoassay 13, 127 (1990).
18.O. D. Velev, K. H. Bhatt, B. G. Prevo, and S. O. Lumsdon, Abstr. Pap. - Am. Chem. Soc. 226, U479 (2003).
21.L. B. Bangs, Am. Clin. Lab. 9, 16 (1990).
22.J. Turkevich, Gold Bull. (Geneva) 18, 125 (1985).
24.P. G. Righetti and T. Caravaggio, J. Chromatogr. A 127, 1 (1976).
25.J. Roth, Techniques in Immunocytochemistry, edited by G. R. Bullock and P. Petrusz (Academic, London, 1982), p. 107.
26.J. E. Beesley, Colloidal Gold: A New Perspective for Cytochemical Marking (Royal Microscopical Society, Oxford, England, 1989), p. 10.
27.X.-L. Sun, X.-L. Zhao, J. Tang, J. Zhou, and F. S. Chu, Int. J. Food Microbiol. 99, 185 (2005).
29.C. Lubelli, A. Chatgilialoglu, A. Bolognesi, P. Strocchi, M. Colombatti, and F. Stirpe, Anal. Biochem. 355, 102 (2006).
30.H. F. Shyu, D. J. Chiao, H. W. Liu, and S. S. Tang, Hybridoma Hybridomics 21, 69 (2002).
32.D. A. Dmitriev, Y. S. Massino, and O. L. Segal, J. Immunol. Methods 280, 183 (2003).
33.M. Stenberg and H. Nygren, J. Immunol. Methods 113, 3 (1988).
34.D. F. Evans and H. Wennerstrom, The Colloidal Domain: Where Physics, Chemistry, Biology, and Technology Meet (Wiley, New York, 1999), pp. 417–428.
40.Radius of ricin is as compared to for IgG. The diffusion rate for the first step is roughly larger than the rate of diffusion for second step. The antibody used to detect ricin is also an immunoglobulin attached onto the gold nanoparticle surface. Hence our assumption of second step to be the rate limiting is still valid and the model explains the dynamics of ricin GOAgg assays.
42.D. A. Handley, Colloidal Gold: Principles, Methods and Applications, edited by M. A. Hayat (Academic, San Diego, 1989), pp. 1.
Article metrics loading...
A novel technique for biomolecular detection in microliter droplets floating on the surface of high density oil is presented. Each droplet was captured and manipulated dielectrophoretically and was used as a site for a microscopic bioassay based on agglutination of antibody-conjugated particles. The results were read out by the pattern of unagglomerated goldnanoparticles collected on the droplet surface. Two formats of bioassays, namely gold only agglutination and gold and latex agglutination, were investigated experimentally by varying analyte concentration, particle size and concentration, number of antigen binding sites per particle, time for incubation, and rate of particle collection on the droplet surface. The microbioassays performance was also evaluated with ricin antibodies and compared to the ricin assays in field use. It is estimated that the droplet based assays require smaller sample volume and are ten times more sensitive, though they require longer times to complete. The experiments were interpreted by modeling the kinetics of particle agglutination and mass transfer processes inside the droplets. The incubation time and antigen concentration values calculated by the model correlate well with the experimental results. The results could allow for development of efficient immunoassays on a chip requiring even smaller sample volumes.
Full text loading...
Most read this month