No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
A transparent cell-culture microchamber with a variably controlled concentration gradient generator and flow field rectifier
3.R. D. Nelson, P. G. Quie, and R. L. Simmons, J. Immunol. 115, 1650 (1975).
5.A. F. Brown, J. Cell Sci. 58, 455 (1982).
7.N. L. Jeon, H. Baskaran, S. K. W. Dertinger, G. M. Whitesides, L. V. D. Water, and M. Toner, Nat. Biotechnol. 20, 826 (2002).
12.J. Diao, L. Young, S. Kim, E. A. Fogarty, S. M. Heilman, P. Zhou, M. L. Shuler, M. Wu, and M. P. DeLisa, Lab Chip 6, 381 (2006).
20.R. Davidsson, A. Boketoft, J. Bristulf, K. Kotarsky, B. Olde, C. Owman, M. Bengtsson, T. Laurell, and J. Emneus, Anal. Chem. 76, 4715 (2004).
23.G. S. Fiorini and D. T. Chiu, Biotechniques 38, 429 (2005).
24.C. L. Ho, T. Y. Mou, P. S. Chiang, C. L. Weng, and N. H. Chow, Biotechniques 38, 267 (2005).
30.J. -Y. Cheng, M. -H. Yen, W. -C. Hsu, and T. -H. Young, in The 8th International Symposium on Laser Precision Microfabrication, LPM2007, Vienna, Austria 2007 (2007).
32.J. Y. Shih, S. C. Yang, T. M. Hong, A. Yuan, J. J. W. Chen, C. J. Yu, Y. L. Chang, Y. C. Lee, K. Peck, C. W. Wu, and P. C. Yang, J. Natl. Cancer Inst. 93, 1392 (2001).
35.D. P. Gaver III and S. M. Kute, Biophys. J. 75, 721 (1998).
Article metrics loading...
Real-time observation of cell growth provides essential information for studies such as cell migration and chemotaxis. A conventional cell incubation device is usually too clumsy for these applications. Here we report a transparent microfluidic device that has an integrated heater and a concentration gradient generator. A piece of indium tin oxide (ITO) coated glass was ablated by our newly developed visible laser-induced backside wet etching (LIBWE) so that transparent heater strips were prepared on the glass substrate. A polymethylmethacrylate (PMMA) microfluidic chamber with flow field rectifiers and a reagent effusion hole was fabricated by a laser and then assembled with the ITO heater so that the chamber temperature can be controlled for cell culturing. A variable chemical gradient was generated inside the chamber by combining the lateral medium flow and the flow from the effusion hole. Successful culturing was performed inside the device. Continuous long-term observation on cell growth was achieved. In this work the flow field, medium replacement, and chemical gradient in the microchamber are elaborated.
Full text loading...
Most read this month