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Design and integration of an all-in-one biomicrofluidic chip
1.C. C. Lee, G. D. Sui, A. Elizarov, C. Y. J. Shu, Y. S. Shin, A. N. Dooley, J. Huang, A. Daridon, P. Wyatt, D. Stout, H. C. Kolb, O. N. Witte, N. Satyamurthy, J. R. Heath, M. E. Phelps, S. R. Quake, and H. R. Tseng, Science 310, 1793 (2005).
8.M. Piatak, Jr., M. S. Saag, L. C. Yang, S. J. Clark, J. C. Kappes, K. C. Luk, B. H. Hahn, G. M. Shaw, and J. D. Lifson, Science 259, 1749 (1993).
9.R. A. Fouchier, T. M. Bestebroer, S. Herfst, L. Van Der Kemp, G. F. Rimmelzwaan, and A. D. Osterhaus, J. Clin. Microbiol. 38, 4096 (2000).
12.M. G. Bergeron, D. B. Ke, C. Ménard, F. J. Picard, M. Gagnon, M. Bernier, M. Ouellette, P. H. Roy, S. Marcoux, and W. D. Fraser, N. Engl. J. Med. 343, 175 (2000).
15.N. R. Beer, E. K. Wheeler, L. L. Houghton, N. Watkins, S. Nasarabadi, N. Hebert, P. Leung, D. W. Arnold, C. G. Bailey, and B. W. Colston, Anal. Chem. 80, 1854 (2008).
23. volume whole reagents containing: (50 ng) DNA template sample YFP-N1, (25 pmole) of each forward and reverse primer, 2X PCR Master Mix (Promega, Madison, WI) and up to . The template (YFP-N1) is a 462bp segment encoding for the first 154 amino acid of eYFP (Accession number AY818378), which is amplified with the forward primer -CGGGATCCCGTGAGCAAGGGCGAGGAGC- and reverse primer -ATTTGCGGCCGCGGCCATGATATAGACGTTGTG-.
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We demonstrate a highly integrated microfluidic chip with the function of DNA amplification. The integrated chip combines giant electrorheological-fluid actuated micromixer and micropump with a microheater array, all formed using soft lithography. Internal functional components are based on polydimethylsiloxane (PDMS) and silver/carbon black-PDMS composites. The system has the advantages of small size with a high degree of integration, high polymerase chain reaction efficiency, digital control and simple fabrication at low cost. This integration approach shows promise for a broad range of applications in chemical synthesis and biological sensing/analysis, as different components can be combined to target desired functionalities, with flexible designs of different microchips easily realizable through soft lithography.
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