1887
banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
oa
Rapid production of protein-loaded biodegradable microparticles using surface acoustic waves
Rent:
Rent this article for
Access full text Article
/content/aip/journal/bmf/3/1/10.1063/1.3055282
1.
1.H. Tamber, P. Johansen, H. P. Merkle, and B. Gander, Adv. Drug Delivery Rev. 57, 357 (2005).
http://dx.doi.org/10.1016/j.addr.2004.09.002
2.
2.M.-A. Benoit, B. Baras, and J. Gillard, Int. J. Pharm. 184, 73 (1999).
3.
3.B. Gander, P. Johansen, H. Nam-Tran, and H. P. Merkle, Int. J. Pharm. 129, 51 (1996).
http://dx.doi.org/10.1016/S0378-5173(99)00109-X
4.
4.V. Coccoli, A. Luciani, S. Orsi, V. Guarino, F. Causa, and P. A. Netti, J. Mater. Sci.: Mater. Med. 19, 1703 (2008).
http://dx.doi.org/10.1007/s10856-007-3253-9
5.
5.T. Morita, Y. Sakamura, Y. Horikiri, T. Suzuki, and H. Yoshino, J. Controlled Release 69, 435 (2000).
http://dx.doi.org/10.1016/S0168-3659(00)00326-6
6.
6.C. Thomasin, P. Johanson, R. Alder, R. Bemsel, G. Hottinger, H. Altorfer, A. D. Wright, E. Wehrli, H. P. Merkle, and B. Gander, Eur. J. Pharm. Biopharm. 42, 16 (1996).
7.
7.Y. Wu and R. L. Clark, J. Colloid Interface Sci. 310, 529 (2007).
http://dx.doi.org/10.1016/j.jcis.2007.02.023
8.
8.I. G. Loscertales, A. Barrero, I. Guerrero, R. Cortijo, M. Marquez, and A. M. Gañán-Calvo, Science 295, 1695 (2002).
http://dx.doi.org/10.1126/science.1067595
9.
9.L. Y. Yeo, Z. Gagnon, and H.-C. Chang, Biomaterials 26, 6122 (2005).
http://dx.doi.org/10.1016/j.biomaterials.2005.03.033
10.
10.L. Y. Yeo and H.-C. Chang, in WIT Trans. Eng. Sci. 52, 223 (2006).
http://dx.doi.org/10.2495/AFM060231
11.
11.G. M. Forde, A. D. Coomes, F. K. Giliam, Y. Han, and M. J. Horsfall, Trans. IChemE Part A 84, 178 (2006).
http://dx.doi.org/10.1205/cherd.05142
12.
12.S. Freitas, H. P. Merkle, and B. Gander, J. Controlled Release 95, 185 (2004).
http://dx.doi.org/10.1016/j.jconrel.2003.11.005
13.
13.H. R. Costantino, L. Firouzabadian, K. Hogeland, C. Wu, C. Beganski, K. G. Carrasquillo, M. Córdova, K. Griebenow, S. E. Zale, and M. A. Tracy, Pharm. Res. 17, 1374 (2000).
http://dx.doi.org/10.1023/A:1007570030368
14.
14.R. M. White and F. W. Volmer, Appl. Phys. Lett. 7, 314 (1965).
http://dx.doi.org/10.1063/1.1754276
15.
15.J. R. Friend, L. Y. Yeo, D. R. Arifin, and A. Mechler, Nanotechnology 19, 145301 (2008).
http://dx.doi.org/10.1088/0957-4484/19/14/145301
16.
16.M. Alvarez, J. R. Friend, and L. Y. Yeo (unpublished).
17.
17.A. Wixforth, C. Strobl, C. Gauer, A. Toegl, J. Scriba, and Z. v Guttenberg, Anal. Bioanal. Chem. 379, 982 (2004).
http://dx.doi.org/10.1007/s00216-004-2693-z
18.
18.L. Y. Yeo and J. Friend, Biomicrofluidics 3, 012002 (2009).
http://dx.doi.org/10.1063/1.3056040
19.
19.M. K. Tan, L. Y. Yeo, and J. R. Friend (unpublished).
20.
20.R. Shilton, M. K. Tan, L. Y. Yeo, and J. R. Friend, J. Appl. Phys. 104, 014910 (2008).
http://dx.doi.org/10.1063/1.2951467
21.
21.H. Li, J. R. Friend, and L. Y. Yeo, Biomed. Microdevices 9, 647 (2007).
http://dx.doi.org/10.1007/s10544-007-9058-2
22.
22.H. Li, J. R. Friend, and L. Y. Yeo, Phys. Rev. Lett. 101, 084502 (2008).
http://dx.doi.org/10.1103/PhysRevLett.101.084502
23.
23.H. Li, J. R. Friend, and L. Y. Yeo, Biomaterials 28, 4098 (2007).
http://dx.doi.org/10.1016/j.biomaterials.2007.06.005
24.
24.A. Qi, J. R. Friend, and L. Y. Yeo, Phys. Fluids 20, 074103 (2008).
http://dx.doi.org/10.1063/1.2953537
25.
25.M. Kurosawa, T. Watanabe, and T. Higuchi, in Proceedings of the IEEE Conference on Micro Electro Mechanical Systems MEMS 1995, Amsterdam, The Netherlands (IEEE, Pistacaway, NJ, 1995), pp. 2530.
26.
26.K. Chono, N. Shimizu, Y. Matsui, J. Kondoh, and S. Shiokawa, Jpn. J. Appl. Phys., Part 1 43, 2987 (2004).
http://dx.doi.org/10.1143/JJAP.43.2987
27.
27.M. Alvarez, J. R. Friend, and L. Y. Yeo, Langmuir 24, 10629 (2008).
http://dx.doi.org/10.1021/la802255b
28.
28.L. Y. Yeo, D. Lastochkin, S.-C. Wang, and H.-C. Chang, Phys. Rev. Lett. 92, 133902 (2004).
http://dx.doi.org/10.1103/PhysRevLett.92.133902
29.
29.J. C. Huang, K. T. Lin, and R. D. Deanin, J. Appl. Polym. Sci. 100, 511 (2002).
30.
30.Z. G. Tang, R. A. Black, J. M. Curran, J. A. Hunt, N. P. Rhodes, and D. F. Williams, Biomaterials 25, 4741 (2004).
http://dx.doi.org/10.1016/j.biomaterials.2003.12.003
31.
31.L. Y. Yeo and J. R. Friend, J. Exp. Nanosci. 1, 177 (2006).
http://dx.doi.org/10.1080/17458080600670015
32.
32.J. P. Hoogenboom, C. Rétif, E. de Bres, M. van de Boer, A. K. van Langen-Suurling, J. Romijn, and A. van Blaaderen, Nano Lett. 4, 205 (2004).
http://dx.doi.org/10.1021/nl034867h
33.
33.J. Raula, H. Eerikainen, and E. Kauppinen, Int. J. Pharm. 284, 13 (2004).
http://dx.doi.org/10.1016/j.ijpharm.2004.07.003
34.
34.K. H. Leong, J. Aerosol Sci. 18, 511 (1987).
http://dx.doi.org/10.1016/0021-8502(87)90066-8
http://aip.metastore.ingenta.com/content/aip/journal/bmf/3/1/10.1063/1.3055282
Loading

Figures

Image of FIG. 1.

Click to view

FIG. 1.

(a) SAW device consisting of a single crystal lithium niobate piezoelectric substrate on which the interdigital transducer (IDT) electrode is patterned. A section of the IDT is enlarged to show the constituent finger pairs. (b) Schematic illustration (lateral view) of the surface acoustic wave propagation along the substrate and its interaction with a sessile liquid droplet. As a consequence of the diffraction of a significant amount of acoustic radiation into the drop at the Rayleigh angle , the drop interface is vigorously destabilized, resulting in its atomization into fine droplets that are ejected from the interface.

Image of FIG. 2.

Click to view

FIG. 2.

(a) Schematic diagram of the experimental setup used for drying and collecting the particles. (b) Representative image of the protein-loaded polymeric particles collected over a glass substrate, taken with an optical microscope. (c) Same image in (b) obtained through fluorescence microscopy.

Image of FIG. 3.

Click to view

FIG. 3.

Representative images of the atomization process acquired through high-speed visualization with a device. In (a), the drop containing the polymer solution is delivered via a syringe pump through the needle and deposited on the piezoelectric substrate. The drop then translates rapidly from left to right in the direction of the SAW propagation; note that the SAWs contain both traveling and standing wave components. As it translates, the drop leaves behind a thin film, which almost cannot be seen. Atomization occurs off the drop interface as well as the thin film. In the latter case, this is responsible for highly ordered regular polymer spot patterns on the substrate.27 In this case, the polymer concentration is 0.2% w/v and the images were captured at . In (b), two successive images capture the process by which droplets are atomized off the interface of the thin trailing film. We note the destabilization of the capillary waves along the interface leads to the formation of a jet, which eventually necks and pinches-off to form the ejected droplet. Given that the images were captured at , the time interval between the two images are approximately . In this case, the polymer concentration is 1% w/v.

Image of FIG. 4.

Click to view

FIG. 4.

Capillary waves formed on the surface of the trailing film comprising the (a) polymer solution , and, (b) polymer/BSA solution , as viewed directly from above. A SAW device is used in this case and the images were acquired through a camera connected to a microscope.

Image of FIG. 5.

Click to view

FIG. 5.

SEM images of a representative sample of BSA-loaded polymer particles and produced with (a) (sample A) and (b) SAW (sample C) devices, for an initial polymer concentration of 0.2% w/v, and their corresponding size distributions.

Image of FIG. 6.

Click to view

FIG. 6.

SEM images of representative samples of BSA-loaded polymer particles produced with (a) (sample B) and (b) (sample D) SAW devices for an initial polymer concentration of 1% w/v.

Image of FIG. 7.

Click to view

FIG. 7.

SEM image of a representative sample of unloaded PCL polymer particles produced with a SAW device for an initial polymer concentration of 1% w/v.

Image of FIG. 8.

Click to view

FIG. 8.

SEM images of two protein-loaded PCL polymer particles produced with a SAW device with an initial polymer concentration of 1% w/v. Image (a) shows a representative particle obtained through the drying tube configuration in Fig. 2(a), whereas image (b) shows a representative particle obtained through inverting the drying tube configuration and reducing the air flow rate.

Image of FIG. 9.

Click to view

FIG. 9.

Planar confocal laser scanning microscopy images taken at vertical positions (a) near the bottom and (b) close to the center of the particles showing the distribution of the fluorescent conjugated BSA within the PCL shell. Image (c) is a three-dimensional reconstruction of a number of these two-dimensional planar scans.

Tables

Generic image for table

Click to view

Table I.

Mean particle diameter for different combinations of the SAW frequency and polymer concentrations employed.

Loading

Article metrics loading...

/content/aip/journal/bmf/3/1/10.1063/1.3055282
2009-01-21
2014-04-25

Abstract

We present a straightforward and rapid surface acoustic wave(SAW) atomization-based technique for encapsulating proteins into order particles composed of a biodegradablepolymeric excipient, using bovine serum albumin (BSA) as an exemplar. Scans obtained from confocal microscopy provide qualitative proof of encapsulation and show the fluorescent conjugated protein to be distributed in a relatively uniform manner within the polymer shell. An ELISA assay of the collected particles demonstrates that the BSA survives the atomization, particle formation, and collection process with a yield of approximately 55%. The SAW atomization universally gave particles with a textured morphology, and increasing the frequency and polymer concentration generally gave smaller particles (to average) with reduced porosity.

Loading

Full text loading...

/deliver/fulltext/aip/journal/bmf/3/1/1.3055282.html;jsessionid=763qeeaj0u1b.x-aip-live-01?itemId=/content/aip/journal/bmf/3/1/10.1063/1.3055282&mimeType=html&fmt=ahah&containerItemId=content/aip/journal/bmf
true
true
This is a required field
Please enter a valid email address
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Rapid production of protein-loaded biodegradable microparticles using surface acoustic waves
http://aip.metastore.ingenta.com/content/aip/journal/bmf/3/1/10.1063/1.3055282
10.1063/1.3055282
SEARCH_EXPAND_ITEM