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Direct measurements of the frequency-dependent dielectrophoresis force
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Figures

Image of FIG. 1.

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FIG. 1.

A diagram depicting the potential well formed by OT. Under an AM DEP force the particle executes an oscillatory motion centered at the point off-set from the center of the optical trap.

Image of FIG. 2.

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FIG. 2.

Schematic of the DEP force spectroscopy setup.

Image of FIG. 3.

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FIG. 3.

(a) The schematic of a DEP quad-electrode and (b) a CCD image of a optically trapped polystyrene particle near the edge of an electrode.

Image of FIG. 4.

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FIG. 4.

Data depicting the linearity of the DEP force as a function of electric field amplitude squared.

Image of FIG. 5.

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FIG. 5.

The DEP force (left) and displacement phase (right) as a function of frequency for four different sizes of polystyrene particles with diameters ranging from .

Image of FIG. 6.

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FIG. 6.

Crossover frequencies as a function of particle size [data shown in squares are from the paper by Green and Morgan (Ref. 8)].

Image of FIG. 7.

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FIG. 7.

(a) The magnitude and (b) phase shift of the particle displacement due to the DEP force for silica and polystyrene spheres.

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/content/aip/journal/bmf/3/1/10.1063/1.3058569
2009-01-02
2014-04-18

Abstract

Dielectrophoresis(DEP), the phenomenon of directed motion of electrically polarizable particles in a nonuniform electric field, is promising for applications in biochemical separation and filtration. For colloidal particles in suspension, the relaxation of the ionic species in the shear layer gives rise to a frequency-dependent, bidirectional DEP force in the radio frequency range. However, quantification methods of the DEP force on individual particles with the pico-Newton resolution required for the development of theories and design of device applications are lacking. We report the use of optical tweezers as a force sensor and a lock-in phase-sensitive technique for analysis of the particle motion in an amplitude modulated DEP force. The coherent detection and sensing scheme yielded not only unprecedented sensitivity for DEP force measurements, but also provided a selectivity that clearly distinguishes the pure DEP force from all the other forces in the system, including electrophoresis, electro-osmosis, heat-induced convection, and Brownian forces, all of which can hamper accurate measurements through other existing methods. Using optical tweezers-based force transducers already developed in our laboratory, we have results that quantify the frequency-dependent DEP force and the crossover frequency of individual particles with this new experimental method.

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Scitation: Direct measurements of the frequency-dependent dielectrophoresis force
http://aip.metastore.ingenta.com/content/aip/journal/bmf/3/1/10.1063/1.3058569
10.1063/1.3058569
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