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Polydimethylsiloxane microfluidic chip with integrated microheater and thermal sensor
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28.The PCR mixture contains DNA template sample YFP-N1, of each forward and reverse primer, 2X PCR Master Mix® (Promega, Madison, WI) and up to . The template (YFP-N1) is a segment encoding for the first 154 amino acid of eYFP (Accession No. AY818378), which is amplified with the forward primer and reverse primer .
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A microheater and a thermal sensor were fabricated inside elastomeric polydimethylsiloxane microchannels by injecting silver paint (or other conductivematerials) into the channels. With a high-precision control scheme, microheaters can be used for rapid heating, with precise temperature control and uniform thermal distribution. Using such a microheater and feedback system, a polymerase chain reaction experiment was carried out whereas the DNA was successfully amplified in , with .
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