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/content/aip/journal/bmf/3/4/10.1063/1.3267098
2009-11-24
2016-12-03

Abstract

Focusing cells into a single stream is usually a necessary step prior to counting and separating them in microfluidic devices such as flow cytometers and cell sorters. This work presents a sheathless electrokinetic focusing of yeast cells in a planar serpentine microchannel using dc-biased acelectric fields. The concurrent pumping and focusing of yeast cells arise from the dcelectrokinetic transport and the turn-induced ac/dc dielectrophoretic motion, respectively. The effects of electric field (including ac to dc field ratio and ac field frequency) and concentration (including buffer concentration and cell concentration) on the cell focusing performance were studied experimentally and numerically. A continuous electrokinetic filtration of E. colicells from yeast cells was also demonstrated via their differential electrokinetic focusing in a serpentine microchannel.

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