NOTICE: Scitation Maintenance Tuesday, May 5, 2015

Scitation will be unavailable on Tuesday, May 5, 2015 between 3:00 AM and 4:00 AM EST due to planned network maintenance.

Thank you for your patience during this process.

banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.
A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans
Rent this article for
Access full text Article
1.E. Braungart, M. Gerlach, P. Riederer, R. Baumeister, and M. C. Hoener, Neurodegener. Dis. 1, 175 (2004).
2.R. Nass, D. M. Miller, and R. D. Blakely, Parkinsonism Relat. Disord. 7, 185 (2001).
3.M. B. Goodman, D. H. Hall, L. Avery, and S. R. Lockery, Neuron 20, 763 (1998).
4.R. Kerr, V. Lev-Ram, G. Baird, P. Vincent, R. Y. Tsien, and W. R. Schafer, Neuron 26, 583 (2000).
5.J. A. Lewis, C. H. Wu, H. Berg, and J. H. Levine, Genetics 95, 905 (1980).
6.J. H. Qin and A. R. Wheeler, Lab Chip 7, 186 (2007).
7.J. M. Gray, D. S. Karow, H. Lu, A. J. Chang, J. S. Chang, R. E. Ellis, M. A. Marletta, and C. I. Bargmann, Nature (London) 430, 317 (2004).
8.K. H. Chung, M. M. Crane, and H. Lu, Nat. Methods 5, 637 (2008).
9.M. M. Crane, K. Chung, and H. Lu, Lab Chip 9, 38 (2009).
10.N. Kim, C. M. Dempsey, J. V. Zoval, J. Y. Sze, and M. J. Madou, Sens. Actuators B 122, 511 (2007).
11.W. W. Shi, J. H. Qin, N. N. Ye, and B. C. Lin, Lab Chip 8, 1432 (2008).
12.S. E. Hulme, S. S. Shevkoplyas, J. Apfeld, W. Fontana, and G. M. Whitesides, Lab Chip 7, 1515 (2007).
13.N. Chronis, M. Zimmer, and C. I. Bargmann, Nat. Methods 4, 727 (2007).
14.T. V. Chokshi, A. Ben-Yakar, and N. Chronis, Lab Chip 9, 151 (2009).
15.S. X. Guo, F. Bourgeois, T. Chokshi, N. J. Durr, M. A. Hilliard, N. Chronis, and A. Ben-Yakar, Nat. Methods 5, 531 (2008).
16.C. B. Rohde, F. Zeng, R. Gonzalez-Rubio, M. Angel, and M. F. Yanik, Proc. Natl. Acad. Sci. U.S.A. 104, 13891 (2007).
17.F. Zeng, C. B. Rohde, and M. F. Yanik, Lab Chip 8, 653 (2008).
18.J. C. McDonald, D. C. Duffy, J. R. Anderson, D. T. Chiu, H. K. Wu, O. J. A. Schueller, and G. M. Whitesides, Electrophoresis 21, 27 (2000).<27::AID-ELPS27>3.0.CO;2-C
19.M. A. Unger, H. P. Chou, T. Thorsen, A. Scherer, and S. R. Quake, Science 288, 113 (2000).
20.S. Brenner, Genetics 77, 71 (1974).

Data & Media loading...


Article metrics loading...



The soil dwelling nematode Caenorhabditis elegans (C. elegans) is an excellent model organism for the study of numerous disease including neurodegenerative disease. In this study, a programmable microvalve-based microfluidic array for real-time and long-term monitoring of the neurotoxin-induced responses of the individual C. elegans was developed. The device consisted of a flow layer and a control layer, which were used for worm manipulation. By activating the programmable microvalves in the control layer, mutiple worms could be individually captured and intermittently immobilized in parallel channels. Thus the mobility behavior, together with the corresponding dopaminergic neuron features of the worms in response to neurotoxin, could be investigated simultaneously. It was found that the neurotoxin enabled to induce mobility defects and dopaminergic neurons loss in worms. The established system is easy and fast to operate, which offers not only the controllable microenvironment for analyzing the individual worms in parallel, monitoring the same worm over time, but also the capability to characterize the mobility behavior and neuron features in response to stimuli simultaneously. In addition, the device enabled to sustain the worm culture over most of their adult lifespan without any harm to worm, providing a potential platform for lifespan and aging research.


Full text loading...

This is a required field
Please enter a valid email address

Oops! This section, does not exist...

Use the links on this page to find existing content.

752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans