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A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans
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1.
1.E. Braungart, M. Gerlach, P. Riederer, R. Baumeister, and M. C. Hoener, Neurodegener. Dis. 1, 175 (2004).
http://dx.doi.org/10.1159/000080983
2.
2.R. Nass, D. M. Miller, and R. D. Blakely, Parkinsonism Relat. Disord. 7, 185 (2001).
http://dx.doi.org/10.1016/S1353-8020(00)00056-0
3.
3.M. B. Goodman, D. H. Hall, L. Avery, and S. R. Lockery, Neuron 20, 763 (1998).
http://dx.doi.org/10.1016/S0896-6273(00)81014-4
4.
4.R. Kerr, V. Lev-Ram, G. Baird, P. Vincent, R. Y. Tsien, and W. R. Schafer, Neuron 26, 583 (2000).
http://dx.doi.org/10.1016/S0896-6273(00)81196-4
5.
5.J. A. Lewis, C. H. Wu, H. Berg, and J. H. Levine, Genetics 95, 905 (1980).
6.
6.J. H. Qin and A. R. Wheeler, Lab Chip 7, 186 (2007).
http://dx.doi.org/10.1039/b613414a
7.
7.J. M. Gray, D. S. Karow, H. Lu, A. J. Chang, J. S. Chang, R. E. Ellis, M. A. Marletta, and C. I. Bargmann, Nature (London) 430, 317 (2004).
http://dx.doi.org/10.1038/nature02714
8.
8.K. H. Chung, M. M. Crane, and H. Lu, Nat. Methods 5, 637 (2008).
http://dx.doi.org/10.1038/nmeth.1227
9.
9.M. M. Crane, K. Chung, and H. Lu, Lab Chip 9, 38 (2009).
http://dx.doi.org/10.1039/b813730g
10.
10.N. Kim, C. M. Dempsey, J. V. Zoval, J. Y. Sze, and M. J. Madou, Sens. Actuators B 122, 511 (2007).
http://dx.doi.org/10.1016/j.snb.2006.06.026
11.
11.W. W. Shi, J. H. Qin, N. N. Ye, and B. C. Lin, Lab Chip 8, 1432 (2008).
http://dx.doi.org/10.1039/b808753a
12.
12.S. E. Hulme, S. S. Shevkoplyas, J. Apfeld, W. Fontana, and G. M. Whitesides, Lab Chip 7, 1515 (2007).
http://dx.doi.org/10.1039/b707861g
13.
13.N. Chronis, M. Zimmer, and C. I. Bargmann, Nat. Methods 4, 727 (2007).
http://dx.doi.org/10.1038/nmeth1075
14.
14.T. V. Chokshi, A. Ben-Yakar, and N. Chronis, Lab Chip 9, 151 (2009).
http://dx.doi.org/10.1039/b807345g
15.
15.S. X. Guo, F. Bourgeois, T. Chokshi, N. J. Durr, M. A. Hilliard, N. Chronis, and A. Ben-Yakar, Nat. Methods 5, 531 (2008).
http://dx.doi.org/10.1038/nmeth.1203
16.
16.C. B. Rohde, F. Zeng, R. Gonzalez-Rubio, M. Angel, and M. F. Yanik, Proc. Natl. Acad. Sci. U.S.A. 104, 13891 (2007).
http://dx.doi.org/10.1073/pnas.0706513104
17.
17.F. Zeng, C. B. Rohde, and M. F. Yanik, Lab Chip 8, 653 (2008).
http://dx.doi.org/10.1039/b804808h
18.
18.J. C. McDonald, D. C. Duffy, J. R. Anderson, D. T. Chiu, H. K. Wu, O. J. A. Schueller, and G. M. Whitesides, Electrophoresis 21, 27 (2000).
http://dx.doi.org/10.1002/(SICI)1522-2683(20000101)21:1<27::AID-ELPS27>3.0.CO;2-C
19.
19.M. A. Unger, H. P. Chou, T. Thorsen, A. Scherer, and S. R. Quake, Science 288, 113 (2000).
http://dx.doi.org/10.1126/science.288.5463.113
20.
20.S. Brenner, Genetics 77, 71 (1974).
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/content/aip/journal/bmf/3/4/10.1063/1.3274313
2009-12-23
2014-10-02

Abstract

The soil dwelling nematode Caenorhabditis elegans (C. elegans) is an excellent model organism for the study of numerous disease including neurodegenerative disease. In this study, a programmable microvalve-based microfluidic array for real-time and long-term monitoring of the neurotoxin-induced responses of the individual C. elegans was developed. The device consisted of a flow layer and a control layer, which were used for worm manipulation. By activating the programmable microvalves in the control layer, mutiple worms could be individually captured and intermittently immobilized in parallel channels. Thus the mobility behavior, together with the corresponding dopaminergic neuron features of the worms in response to neurotoxin, could be investigated simultaneously. It was found that the neurotoxin enabled to induce mobility defects and dopaminergic neurons loss in worms. The established system is easy and fast to operate, which offers not only the controllable microenvironment for analyzing the individual worms in parallel, monitoring the same worm over time, but also the capability to characterize the mobility behavior and neuron features in response to stimuli simultaneously. In addition, the device enabled to sustain the worm culture over most of their adult lifespan without any harm to worm, providing a potential platform for lifespan and aging research.

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Scitation: A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans
http://aip.metastore.ingenta.com/content/aip/journal/bmf/3/4/10.1063/1.3274313
10.1063/1.3274313
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