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Optofluidic in situ maskless lithography of charge selective nanoporous hydrogel for DNA preconcentration
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View: Figures


Image of FIG. 1.
FIG. 1.

Microfluidic channel design for charge selective preconcentration using negatively charged hydrogel, poly-AMPS. Charged sample flows in the main channel (S-D) and potassium phosphate buffer is filled in polymer channel (P).

Image of FIG. 2.
FIG. 2.

(a) Schematic diagram of proximity lithography. (b) Schematic diagram of projection lithography using DMD device as a dynamic mask.

Image of FIG. 3.
FIG. 3.

Schematic illustration of instrumentation for charge selective preconcentration. A negatively charged hydrogel is produced by OFML system. Spatial light modulator (DMD) inside the system produces arbitrary UV light pattern dynamically.

Image of FIG. 4.
FIG. 4.

Calculated intensity of light passing through the contact mask (red line: mask opening with feature size of , green line: Fresnel diffraction pattern at the distance of 1 mm from the mask).

Image of FIG. 5.
FIG. 5.

3D optical characterization of a negatively charged hydrogel produced by the OFML system.

Image of FIG. 6.
FIG. 6.

3D characterization of sample preconcentration. Negatively charged fluorescein molecule is used for analysis. (a) Time dependent sample preconcentration profile. (b) 3D confocal microscope image after 1 h of preconcentration. (c) Top view of preconcentration profile (b). (d) Cross sectional side view of (b).

Image of FIG. 7.
FIG. 7.

Preconcentration profile of DNA. Cy-3 labeled 15 bp ssDNAs are concentrated in the region near the ion-depletion region. (a) Time dependent sample preconcentration profile. (b) Fluorescence intensity across the main microchannel (S-D). Distance is referenced to the left corner of the poly-AMPS plug. (c) (left -axis) Integrated fluorescence intensities over the entire preconcentrated plugs, (right -axis) peak fluorescence intensities of preconcentrated plugs.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Optofluidic in situ maskless lithography of charge selective nanoporous hydrogel for DNA preconcentration