Microfluidic channel design for charge selective preconcentration using negatively charged hydrogel, poly-AMPS. Charged sample flows in the main channel (S-D) and potassium phosphate buffer is filled in polymer channel (P).
(a) Schematic diagram of proximity lithography. (b) Schematic diagram of projection lithography using DMD device as a dynamic mask.
Schematic illustration of instrumentation for charge selective preconcentration. A negatively charged hydrogel is produced by OFML system. Spatial light modulator (DMD) inside the system produces arbitrary UV light pattern dynamically.
Calculated intensity of light passing through the contact mask (red line: mask opening with feature size of , green line: Fresnel diffraction pattern at the distance of 1 mm from the mask).
3D optical characterization of a negatively charged hydrogel produced by the OFML system.
3D characterization of sample preconcentration. Negatively charged fluorescein molecule is used for analysis. (a) Time dependent sample preconcentration profile. (b) 3D confocal microscope image after 1 h of preconcentration. (c) Top view of preconcentration profile (b). (d) Cross sectional side view of (b).
Preconcentration profile of DNA. Cy-3 labeled 15 bp ssDNAs are concentrated in the region near the ion-depletion region. (a) Time dependent sample preconcentration profile. (b) Fluorescence intensity across the main microchannel (S-D). Distance is referenced to the left corner of the poly-AMPS plug. (c) (left -axis) Integrated fluorescence intensities over the entire preconcentrated plugs, (right -axis) peak fluorescence intensities of preconcentrated plugs.
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