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Passive optical separation and enrichment of cells by size difference
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Image of FIG. 1.
FIG. 1.

(a) Processing steps for fabricating the PDMS microchannels and the two level sorting device. (b) The bonding procedure for the final device.

Image of FIG. 2.
FIG. 2.

Schematic of optical sorting microfluidic system. The focused laser was applied at the junction of the two overlapping channels.

Image of FIG. 3.
FIG. 3.

Size distribution of yeast cells before separation. We grouped these cells into two groups: small cells (diameter , 43.4%) and large cells (diameter , 56.6%).

Image of FIG. 4.
FIG. 4.

Sequential images of yeast cell when passing through the intersection. (a) Small (white arrows, 1 and 2) and large (yellow arrows, 3, 4, and 5) yeast cells initially flowing in the bottom channel. (b) When they encounter the line laser (dashed box), the large yeast cell was stopped and pushed to upper channel while the small yeast cell passed straight through. (c) and (d) The cells that are moving to the top channel become out of focus and disappear from the image when it is totally switched to top channel. The large cell and small cell are successfully separated and are readily collected at two different outlets. The sorting box was in size. The cells were moving at speeds of . The blue arrows show the direction of fluid flow (enhanced online). [URL: http://dx.doi.org/10.1063/1.3523057.1]10.1063/1.3523057.1

Image of FIG. 5.
FIG. 5.

(a) Trajectories of large cells (diameter ) and (b) small cells (diameter ). The dashed box indicates the line laser and white arrows show the direction of fluid flow. Cells are moving at .

Image of FIG. 6.
FIG. 6.

(a) Composition ratio of cells collected in lower outlet. (b) Composition ratio of cells collected in upper outlet. Cells were grouped into larger and smaller than in diameter.



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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Passive optical separation and enrichment of cells by size difference