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Polyelectrolyte multilayer surface functionalization of poly(dimethylsiloxane) (PDMS) for reduction of yeast cell adhesion in microfluidic devices
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10.1063/1.3523059
/content/aip/journal/bmf/4/4/10.1063/1.3523059
http://aip.metastore.ingenta.com/content/aip/journal/bmf/4/4/10.1063/1.3523059

Figures

Image of FIG. 1.
FIG. 1.

Scheme of the parallel flow channel used in this study (bottom: sectional view; top: channel profile).

Image of FIG. 2.
FIG. 2.

(a) FTIR-ATR spectra of the investigated PEM coatings on stainless steel before PEGylation (curves are arranged according to the order of the labeling). (b) FTIR-ATR spectra of PEM coatings on stainless steel after adsorption of PAA(200)-5-PEG(5) copolymer (spectra of PEMs on stainless steel foil were subtracted).

Image of FIG. 3.
FIG. 3.

(a) Relative number of remaining cells as a function of the wall shear stress for PDADMAC/PAA coatings. (b) Relative number of remaining cells as a function of the wall shear stress for PEM coatings assembled at PAH/PAA solution pH of 7.5/3.5. (c) Relative number of remaining cells as a function of the wall shear stress for a PEGylated PEM compared to PEG-silane modified PDMS (in the case of PEG-silane the experiment was repeated twice instead of three times).

Image of FIG. 4.
FIG. 4.

Absolute number of remaining cells after application of for all PDMS surface modifications investigated in this study (coating numbers according to Table I). Same colors refer to the same type of PEM. No hatching: PEMs terminating with negatively charged polyelectrolyte . Cross-hatched: PEMs terminating with positively charged polyelectrolyte . Striped: PEMs capped with PAA(200)-5-PEG(5) or PEG-silane.

Image of FIG. 5.
FIG. 5.

Value of (based on linear interpolation) for all PDMS surface modifications investigated in this study (coating numbers according to Table I). Same colors refer to the same type of PEM. No hatching: PEMs terminating with negatively charged polyelectrolyte . Cross-hatched: PEMs terminating with positively charged polyelectrolyte . Striped: PEMs capped with PAA(200)-5-PEG(5) or PEG-silane.

Tables

Generic image for table
Table I.

Coatings on PDMS used for adhesion experiments with yeast S. cerevisiae DSM 2155.

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/content/aip/journal/bmf/4/4/10.1063/1.3523059
2010-12-29
2014-04-17
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Polyelectrolyte multilayer surface functionalization of poly(dimethylsiloxane) (PDMS) for reduction of yeast cell adhesion in microfluidic devices
http://aip.metastore.ingenta.com/content/aip/journal/bmf/4/4/10.1063/1.3523059
10.1063/1.3523059
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