Concentrations (, , represented by a solid, a dotted, and a dashed-dotted line, respectively) and electric field in the transition zone region according to Eqs. (8)–(10). The parameters chosen are listed in Table I. The vertical lines correspond to .
Minimal diameter of beads transported along with the ITP transition zone according to Eqs. (17) and (16) and for the parameters given in Table I [however, was calculated according to Eqs. (1) and (5), and was varied]. The solid line corresponds to and the dashed one to .
Schematic operation of the bead collector. As the ITP transition zone sweeps along the channel, beads dispersed in the LE are collected and move along with the zone.
Bead separation. Two different types of beads are dispersed in the LE at the start of the experiment. In this case, only one type is swept along with the transition zone, while the other is left behind in the TE.
Cross-channel chip with filling options for the reservoirs.
Collected beads at the transition zone interface after (a) 15 s, (b) 60 s, and (c) 120 s. The applied voltage is 800 V. The movie shows capture and transport of diameter beads at the ITP transition zone at an applied voltage of 800 V. The bead movement is from left to right and starts at a position close to the injection cross and ends close to the LE reservoir. The camera position is manually adjusted in order to track the bead cluster (enhanced online). [URL: http://dx.doi.org/10.1063/1.3555194.1]10.1063/1.3555194.1
Velocity of the particle cluster as a function of position in the channel at a voltage of 800 V.
Bead cluster at (from top to bottom) 2000, 1000, 500, and 300 V at positions 5 mm (left) and 20 mm (right) behind the injection cross.
Position of the beads and a fluorescent dye (Alexa 488) as a function of time for a voltage of . Error bars indicate the standard deviation from three measurements.
Gap between Alexa (dark region) and beads before (top group) and after (bottom group) addition of at a position of 10, 15, and 20 mm (from top to bottom) along the channel.
Separation of beads from beads at the transition zone after 18 s (top) and 210 s (bottom). The applied voltage is .
Simultaneous transport of 5 and beads at the transition zone (a) after 23 s and (b) after 90 s. The applied voltage is . The movie shows the transport and demonstrates the considerable internal motion that is observed within the cluster. From time to time, the camera position is manually adjusted in order to track the bead cluster moving from left to right (enhanced online). [URL: http://dx.doi.org/10.1063/1.3555194.2]10.1063/1.3555194.2
Parameters used for the test case.
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