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Transport and separation of micron sized particles at isotachophoretic transition zones
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10.1063/1.3555194
/content/aip/journal/bmf/5/1/10.1063/1.3555194
http://aip.metastore.ingenta.com/content/aip/journal/bmf/5/1/10.1063/1.3555194

Figures

Image of FIG. 1.
FIG. 1.

Concentrations (, , represented by a solid, a dotted, and a dashed-dotted line, respectively) and electric field in the transition zone region according to Eqs. (8)–(10). The parameters chosen are listed in Table I. The vertical lines correspond to .

Image of FIG. 2.
FIG. 2.

Minimal diameter of beads transported along with the ITP transition zone according to Eqs. (17) and (16) and for the parameters given in Table I [however, was calculated according to Eqs. (1) and (5), and was varied]. The solid line corresponds to and the dashed one to .

Image of FIG. 3.
FIG. 3.

Schematic operation of the bead collector. As the ITP transition zone sweeps along the channel, beads dispersed in the LE are collected and move along with the zone.

Image of FIG. 4.
FIG. 4.

Bead separation. Two different types of beads are dispersed in the LE at the start of the experiment. In this case, only one type is swept along with the transition zone, while the other is left behind in the TE.

Image of FIG. 5.
FIG. 5.

Cross-channel chip with filling options for the reservoirs.

Image of FIG. 6.
FIG. 6.

Collected beads at the transition zone interface after (a) 15 s, (b) 60 s, and (c) 120 s. The applied voltage is 800 V. The movie shows capture and transport of diameter beads at the ITP transition zone at an applied voltage of 800 V. The bead movement is from left to right and starts at a position close to the injection cross and ends close to the LE reservoir. The camera position is manually adjusted in order to track the bead cluster (enhanced online). [URL: http://dx.doi.org/10.1063/1.3555194.1]10.1063/1.3555194.1

Image of FIG. 7.
FIG. 7.

Velocity of the particle cluster as a function of position in the channel at a voltage of 800 V.

Image of FIG. 8.
FIG. 8.

Bead cluster at (from top to bottom) 2000, 1000, 500, and 300 V at positions 5 mm (left) and 20 mm (right) behind the injection cross.

Image of FIG. 9.
FIG. 9.

Position of the beads and a fluorescent dye (Alexa 488) as a function of time for a voltage of . Error bars indicate the standard deviation from three measurements.

Image of FIG. 10.
FIG. 10.

Gap between Alexa (dark region) and beads before (top group) and after (bottom group) addition of at a position of 10, 15, and 20 mm (from top to bottom) along the channel.

Image of FIG. 11.
FIG. 11.

Separation of beads from beads at the transition zone after 18 s (top) and 210 s (bottom). The applied voltage is .

Image of FIG. 12.
FIG. 12.

Simultaneous transport of 5 and beads at the transition zone (a) after 23 s and (b) after 90 s. The applied voltage is . The movie shows the transport and demonstrates the considerable internal motion that is observed within the cluster. From time to time, the camera position is manually adjusted in order to track the bead cluster moving from left to right (enhanced online). [URL: http://dx.doi.org/10.1063/1.3555194.2]10.1063/1.3555194.2

Tables

Generic image for table
Table I.

Parameters used for the test case.

video/mp4,video/x-flv,video/flv,audio.mp3,audio.mpeg

Multimedia

The following multimedia file is available, if you log in: 1.3555194.original.v1.avi
The following multimedia file is available, if you log in: 1.3555194.original.v2.avi
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/content/aip/journal/bmf/5/1/10.1063/1.3555194
2011-03-23
2014-04-20
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Transport and separation of micron sized particles at isotachophoretic transition zones
http://aip.metastore.ingenta.com/content/aip/journal/bmf/5/1/10.1063/1.3555194
10.1063/1.3555194
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