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Thiolene-based microfluidic flow cells for surface plasmon resonance imaging
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Image of FIG. 1.
FIG. 1.

(a) The fabricated microfluidic devices with dime-sized imaging windows. (b) Mask design (top) and SPR image (bottom) of straight channels. The SPR image compares channels filled with water (left) to isopropanol (right). The difference in pixel intensity is due to the refractive index mismatch of two solutions. (c) Mask design (top) and SPR image (bottom) of serpentine channel. SPR image was taken using PBS. The width of channels in (b) and (c) is ; the thickness is .

Image of FIG. 2.
FIG. 2.

[(a)–(e)] Thiolene microfluidic device fabrication scheme. Transparency printed mask was taped to a BK7 glass slide before being placed onto thiolene filled PDMS reactor. Exposure to UV light photopolymerized the thiolene resin and formed the microfluidic channels. A gold-coated SF10 slide was used to cap the channels. (f) The experimental setup followed the Kretschmann configuration where the SF10 prism and device were coupled using refractive index matching fluid. Images are collected using a CCD camera.

Image of FIG. 3.
FIG. 3.

(a) Streptavidin binding to biotin functionalized monolayers on glass surface. SPR images (b) and difference images (c) taken before and after streptavidin flow in the recycle mode. Unbound streptavidin was removed by PBS rinse at 65 min. (d) A low concentration of streptavidin (150 nM) injected over biotin functionalized monolayers on gold surface. Unbound streptavidin was removed by PBS rinse at 20 min.


Generic image for table
Table I.

A swelling ratio describes the amount of swelling or delamination due to solvent-thiolene interaction, a value of 1 denotes no change. denotes microfluidic channel swelling after 2 h. , solvent swelling on 2 mm squares of thiolene after 24 h. Thiolene swelling ratios are compared to PDMS’s swelling ratio previously reported, , for 24 h. For channel swelling, , the initial width is larger than the final width, resulting in values less than unity.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Thiolene-based microfluidic flow cells for surface plasmon resonance imaging