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Bromo-oxidation reaction in enzyme-entrapped alginate hollow microfibers
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Image of FIG. 1.
FIG. 1.

(a) Schematic of hollow microfiber generation using a two-channel microfluidic device. (b) Photograph of the microfluidic device showing generation of alginate microfibers composed of 1% (w/v) Na-alginate mixed with blue dye. (c) Enzyme-entrapped alginate hollow fibers.

Image of FIG. 2.
FIG. 2.

Absorbance spectra of conversion of phenol red to bromophenol blue in the bulk reaction.

Image of FIG. 3.
FIG. 3.

(a) Fluorescence micrograph of BSA-FITC-immobilized alginate hollow fiber by using Z-stack scanning. (b) Fluorescence image of alginate hollow fiber showing bore of the fiber. (c) SEM image of a dried alginate hollow fiber. (d) Alginate fiber with brominated product.

Image of FIG. 4.
FIG. 4.

Enzyme-entrapped alginate hollow microfibers embedded in a PDMS matrix. (a) Fabrication schematic. (b) Photograph of working device.

Image of FIG. 5.
FIG. 5.

Reaction showing transformation of Phenol Red to Bromophenol Blue catalyzed by vanadium bromoperoxidase.

Image of FIG. 6.
FIG. 6.

Effect of chitosan coating on alginate hollow microfiber. (a) A bare alginate hollow microfiber with diffused GFP. (b) Hollow microfiber with chitosan coating of inner wall showing no evidence of inwards diffusion of GFP; diffusion of phenol red in to the bare alginate matrix. [(c) and (d)] From alginate hollow fiber coated with chitosan.


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Table I.

Comparative yields of Bromophenol Blue using enzyme-loaded alginate hollow microfiber with and without chitosan coating.


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Scitation: Bromo-oxidation reaction in enzyme-entrapped alginate hollow microfibers