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A microdevice for the creation of patent, three-dimensional endothelial cell-based microcirculatory networks
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10.1063/1.3609264
/content/aip/journal/bmf/5/3/10.1063/1.3609264
http://aip.metastore.ingenta.com/content/aip/journal/bmf/5/3/10.1063/1.3609264
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Schematic of the microvascular network design.

Image of FIG. 2.
FIG. 2.

Measured superficial velocities from horizontal and vertical velocity profiles compared to calculated superficial velocity. Standard error bars are smaller than the data symbols.

Image of FIG. 3.
FIG. 3.

Horizontal-plane velocity profiles and derived shear rates at an inlet volumetric flow rate of 2 μl min−1 for microchannels in the microvascular microdevice. Distance from the centreplane is along the horizontal plane, from side to side, of channels. Standard error bars are smaller than the data symbols.

Image of FIG. 4.
FIG. 4.

Vertical-plane velocity profiles and derived shear rates at an inlet volumetric flow rate of 2 μl min−1 for microchannels in the microvascular microdevice. Distance from the centreplane is along the vertical plane, from top to bottom, of channels. Standard error bars are smaller than the data symbols.

Image of FIG. 5.
FIG. 5.

Photomicrographs of HUVECs in microvascular microdevice after 24 h perfusion at an inlet flow rate of 24 μl min−1. (a) HUVECs appeared to form an even confluent layer throughtout all five microchannels. Scale bar: 100 μm. (b), (c) HUVECs lined corners of the microvascular network. Scale bar: 50 μm.

Image of FIG. 6.
FIG. 6.

Tilted 3D images of HUVECs at sections of the microvascular network. HUVECs were immunostained for von Willebrand factor (red), actin stress fibres (green), and nuclei (blue) and found to line all walls and corners of all channels. Von Willebrand factor is observed in all cells, but at different depths. Images only show von Willebrand factor at that plane of view. Since the 3D images are tilted, scale bars are provided only as estimation. Scale bars are shown as yellow arrows in x, y, and z axes. (a) Schematic to show where sections (b)-(e) are located on the overall network. (b) Corner of the network. Scale bar: 50 μm. (c) Between two channels. Scale bar: 100 μm. (d) Entrance into a channel. Scale bar: 50 μm. (e) Microchannels. Scale bars: 100 μm.

Image of FIG. 7.
FIG. 7.

HUVEC lining all microchannels. (a)-(d) HUVEC were immunostained for von Willebrand factor (red), actin stress fibres (green), and nuclei (blue). HUVECs lined all four sides of the channels as shown in vertical cross-sectional (a), top horizontal cross-sectional (b), middle horizontal cross-sectional (c), and bottom horizontal cross-sectional (d) views of the channels. Scale bars: 50 μm. (e) The HUVEC monolayer was immunostained for VE-cadherin (green), von Willebrand factor (red), and nuclei (blue). Green and red channels overlap to give yellow. Scale bars: 50 μm.

Image of FIG. 8.
FIG. 8.

The HUVEC monolayer was immunostained for VE-cadherin (green), von Willebrand factor (red), and nuclei (blue). Green and red channels overlap to give yellow. Scale bar, 50 μm.

Image of FIG. 9.
FIG. 9.

Quantitative analysis of cell morphology for HUVECs after 24 h perfusion. (a) Cell size of HUVECs after 24 h of perfusion; (b) cell perimeter of HUVECs after 24 h of perfusion; (c) cell circularity of HUVECs after 24 h of perfusion. A circularity value of 1.0 indicates a perfect circle, as the value approaches 0.0 it indicates an increasingly elongated polygon. Data are the mean ± SE.

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/content/aip/journal/bmf/5/3/10.1063/1.3609264
2011-08-16
2014-04-19
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: A microdevice for the creation of patent, three-dimensional endothelial cell-based microcirculatory networks
http://aip.metastore.ingenta.com/content/aip/journal/bmf/5/3/10.1063/1.3609264
10.1063/1.3609264
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