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Nanofiber-modified surface directed cell migration and orientation in microsystem
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10.1063/1.3614457
/content/aip/journal/bmf/5/3/10.1063/1.3614457
http://aip.metastore.ingenta.com/content/aip/journal/bmf/5/3/10.1063/1.3614457
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Diagram depicting the process for patterning PDMS surface via electrospun nanofibers.

Image of FIG. 2.
FIG. 2.

The photographs of the electrospun PVP nanofibers and produced patterned PDMS surfaces under different concentrations. (a) The microscope images of the PVP nanofibers spun with five concentrations (640×). SEM image of PVP nanofibers spun with 16% initial concentration. (b) The microscope images of PDMS surfaces embedded with PVP nanofibers (640×). (c) The microscope images of patterned PDMS surfaces after fibers removal (640×). SEM image of PDMS surfaces patterned with microstructures.

Image of FIG. 3.
FIG. 3.

The designed microdevice for cell behavior investigation by integrating microfluidic channel with PDMS substrate sacrificed by nanofibers. (a) The illustration of the microdevice for investigating cell migration on patterned surfaces. (b) The schematic of microdevice for investigating cell orientation under combined effects of microchannel spatial confinement and patterned surface guidance.

Image of FIG. 4.
FIG. 4.

Characterization of cell viability and proliferation on PDMS surfaces by using PVP nanofibers sacrificed method. (a) The images of cell morphology on surfaces that patterned with different diameters of PVP nanofibers (100×). (b) Cell proliferation on patterned surfaces by counting cell number (***P < 0.001, *P < 0.05).

Image of FIG. 5.
FIG. 5.

Cell migration investigation used microdevice by integrating microfluidic channel with nanofiber patterned surface. (a) The real-time images of cell migration in the microchannels which are parallel with the patterned surface (100×). (b) The fluorescent microscope images of cell migration in the microchannels that are parallel or perpendicular with patterned surface (100×). (c) The comparison of migrated cells number in microfluidic channels (*P < 0.05).

Image of FIG. 6.
FIG. 6.

The immunofluorescent staining images of cell orientation on patterned (a) and unpatterned surfaces (b) (100×) (red: F-actin, blue: nuclei stained with DAPI).

Image of FIG. 7.
FIG. 7.

Cell orientation under combined effects of microchannel spatial confinement and nanofiber patterned surface guidance. (a) The fluorescent images of cell orientation within diverse diameter microchannels with different angle on the microfiber patterned surface. (100×) (red solid lines represent patterned surface orientation). (b) The decisive factor of cells orientation in complicated microenvironments. (N1: the cell number which is counted having the same orientation with patterned surfaces in confined microchannel; N2: the total cell number in confined microchannel; N3: the cell number which is counted having the same orientation with the microchannel).

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/content/aip/journal/bmf/5/3/10.1063/1.3614457
2011-09-20
2014-04-23
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Nanofiber-modified surface directed cell migration and orientation in microsystem
http://aip.metastore.ingenta.com/content/aip/journal/bmf/5/3/10.1063/1.3614457
10.1063/1.3614457
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