Schematic illustration of the fabrication process used to integrate the PSi array with a PDMS microfluidic system: (a) plasma activation of the surfaces; (b) sealed device; (c) schematic of the microfluidics.
(a) Top and (b) lateral views of the microfluidic assisted porous silicon array.
Reflectivity spectra of a PSi element before (solid line) and after (dashed line) the sealing with the PDMS microfluidic system.
Top image: optical micrograph of a microfluidic channel. Bottom image: reflectivity spectra of the PSi elements before (solid line) and after (dashed line) the DNA probe functionalization.
DNA probes bound to the porous silicon surface calculated for different values of the association rate, kon .
Comparison between the DNA concentration distribution in the first (a) and last (b) microchambers just after the injection of the probe. A significant difference in DNA probes concentration is well evident. The numerical simulation has been obtained with an inlet velocity of 3 • 10−3 m/s.
Maps of DNA concentration distribution in the first (a) and last (b) microchambers together with the mean concentration variations versus the length and the depth of the channel. The data have been calculated using an inlet velocity of 3 • 10−2 m/s.
Reflectivity spectra of a PSi element before (solid line) and after (dashed line) the DNA probe attachment, and after (dotted line) the hybridization with the complementary DNA.
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