1887
banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
Influences of electric field on living cells in a charged water-in-oil droplet under electrophoretic actuation
Rent:
Rent this article for
USD
10.1063/1.3665222
/content/aip/journal/bmf/5/4/10.1063/1.3665222
http://aip.metastore.ingenta.com/content/aip/journal/bmf/5/4/10.1063/1.3665222
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Schematic view of the experimental setup. The time step between sequential images of a 2.0 μl cell suspension droplet is 0.12 s.

Image of FIG. 2.
FIG. 2.

Trypan blue test results. (a) Control group (without any treatment). (b) Silicone oil group (Silicone oil in 1 h). (c) Experimental group under 3 kV/cm for 90 s. (d) Effects of silicone oil and ECD actuation on fibroblast viability (control group: 94.7% ± 0.59%, silicone group: 93.8% ± 1.2%, and experimental group: 94.0% ± 2.1%).

Image of FIG. 3.
FIG. 3.

Phase contrast microscopic images for proliferated fibroblasts under different experimental conditions at different days. Colors are added artificially to distinguish columns easily.

Image of FIG. 4.
FIG. 4.

Influence of silicone oil on the proliferation of fibroblast. The average number of cells in sample images at day 1 is 41.5 ± 15.8 for the control group and 72 ± 9.3 for the silicone oil group and at day 6, it is 1895.7 ± 393 for the control group and 2578.7 ± 322.4 for the silicone oil group, respectively.

Image of FIG. 5.
FIG. 5.

Influence of the ECD actuation on the proliferation of fibroblast. (a) The effect of electric field strength. (b) The effect of ECD actuation time.

Image of FIG. 6.
FIG. 6.

The dependency of the proliferation rate of fibroblast at day 6 on the number of cells at day 1.

Image of FIG. 7.
FIG. 7.

The effects of cell concentration on the viability and proliferation of fibroblast. (a) The viability test results for 2.5 × 105 cells/ml. The P value of the t test is 0.49 (control group: 93.2% ± 5.8%, experimental group: 90.5% ± 6.1%). (b) The viability test results for 5.0 × 105 cells/ml. The P value of the t test is 0.62 (control group: 90.2% ± 8.0%, experimental group: 92.7% ± 7.2%). (c) The proliferation test results for 2.5 × 105 cells/ml. The P value of the t test with the control group is 0.88 for the experimental group and 0.16 for the silicone oil group. (d) The proliferation test results for 5.0 × 105 cells/ml. The P value of the t test with the control group is 0.42 for the experimental group and 0.83 for the silicone oil group.

Loading

Article metrics loading...

/content/aip/journal/bmf/5/4/10.1063/1.3665222
2011-12-02
2014-04-17
Loading

Full text loading...

This is a required field
Please enter a valid email address
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Influences of electric field on living cells in a charged water-in-oil droplet under electrophoretic actuation
http://aip.metastore.ingenta.com/content/aip/journal/bmf/5/4/10.1063/1.3665222
10.1063/1.3665222
SEARCH_EXPAND_ITEM