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Growth propagation of yeast in linear arrays of microfluidic chambers over many generations
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10.1063/1.3668243
/content/aip/journal/bmf/5/4/10.1063/1.3668243
http://aip.metastore.ingenta.com/content/aip/journal/bmf/5/4/10.1063/1.3668243
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Figures

Image of FIG. 1.
FIG. 1.

Schematic illustration (a), layout (b), and fabrication process (c) of the device for investigating the growth propagation of yeast in linear arrays of micro-chambers. Cells are first loaded into the up-most chambers and then grow sequentially along the linear arrays (blue). Nutrient is provided by diffusion from a multi-U shaped channel (red). The first level of the mold for soft lithography is defined by photolithography, lift-off, and reactive ion etching techniques on the surface of a silicon wafer. The second level is patterned by photolithography with a SU8 resist. After molding by soft lithography, the PDMS layer is irreversibly bonded to a cover glass.

Image of FIG. 2.
FIG. 2.

Yeast growth in a liner array of 50 × 50 μm2 square chambers with 6 μm wide and 80 μm long connection channels. (a) DIC image of the last three occupied chambers. (b) Corresponding fluorescence image of MYO1-GFP protein. (c) Corresponding fluorescence image of MCM-mCherry protein.

Image of FIG. 3.
FIG. 3.

Variation of the cell number as a function of culture time in one of the micro-chambers. Cells are sub-sequentially loaded into the chamber from a 6 μm wide and 40 μm long connection channel. The solid line is a fitting of the experimental data with a homogenous loading of 28 cells during 7 h. The dashed line is obtained with only one cell input.

Image of FIG. 4.
FIG. 4.

Variation of the cell number as a function of culture time in a chamber with 12 μm wide and 20 μm long inter-connection channels. The solid line is a fitting with a homogeneous loading of 8 cells and two additional loading of 28 cells at 13 min and 30 cells at 75 min. The dashed line is obtained with a homogeneous cell loading after an initial loading of 28 cells at 13 min.

Image of FIG. 5.
FIG. 5.

Variation of the number of occupied chambers as a function of culture time for linear arrays of the same chamber size but different geometries of connection channels.

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/content/aip/journal/bmf/5/4/10.1063/1.3668243
2011-12-16
2014-04-20
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Growth propagation of yeast in linear arrays of microfluidic chambers over many generations
http://aip.metastore.ingenta.com/content/aip/journal/bmf/5/4/10.1063/1.3668243
10.1063/1.3668243
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