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The effects of laser welding on heterogeneous immunoassay performance in a microfluidic cartridge
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10.1063/1.3668261
/content/aip/journal/bmf/5/4/10.1063/1.3668261
http://aip.metastore.ingenta.com/content/aip/journal/bmf/5/4/10.1063/1.3668261

Figures

Image of FIG. 1.
FIG. 1.

The principle of laser transmission welding.

Image of FIG. 2.
FIG. 2.

Microfluidic cartridge. A photograph (left) of an injection molded polystyrene plate, which is sealed with a black polystyrene plate using laser welding. A schematic of the cartridge structure (right), with red arrow pointing to the reaction chamber. (1) Buffer channel, (2) sample channel, (3) pressure channel, (4) passive valves (arrows), (5) mixer, (6) mixing channel, (7) washing chamber, (8) reaction chamber, and (9) waste chamber.

Image of FIG. 3.
FIG. 3.

A schematic drawing of the laser welding setup.

Image of FIG. 4.
FIG. 4.

Stereo microscope images from top direction of the welding seam using 3.15× and 64× zoom.

Image of FIG. 5.
FIG. 5.

(a) For the temperature measurements, the cartridges were cut in half. The dashed red line represents the cutting line. (b) A schematic drawing of the test arrangement in the channel temperature measurements.

Image of FIG. 6.
FIG. 6.

TSH-immunoassay in cartridges sealed either with an adhesive tape or using laser welding (2 × 4 scanning points), TSH concentration versus background-corrected—counts per second (cps).

Image of FIG. 7.
FIG. 7.

The maximum channel temperatures during contour welding with 50 mm/s speed and 1.6 W laser power.

Image of FIG. 8.
FIG. 8.

Thermal image captured 0.13 s after the beam has passed the line LI01 using a 100 μm welding distance between the seam and the channel wall.

Image of FIG. 9.
FIG. 9.

Thermal image captured 3 s after the beam has passed the line LI01 using 100 μm welding distance between the seam and the channel wall. Temperature at the bottom of the channel is near to its maximum value.

Tables

Generic image for table
Table I.

Average fluorescence signals and coefficients of variance from model immunoassays for reaction chambers of the cartridges sealed either with an adhesive tape or by the use of laser welding.

Generic image for table
Table II.

Signal-to-background ratios for all TSH concentrations for the cartridges sealed with an adhesive tape and by use of laser welding.

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/content/aip/journal/bmf/5/4/10.1063/1.3668261
2011-12-12
2014-04-23
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: The effects of laser welding on heterogeneous immunoassay performance in a microfluidic cartridge
http://aip.metastore.ingenta.com/content/aip/journal/bmf/5/4/10.1063/1.3668261
10.1063/1.3668261
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