The principle of laser transmission welding.
Microfluidic cartridge. A photograph (left) of an injection molded polystyrene plate, which is sealed with a black polystyrene plate using laser welding. A schematic of the cartridge structure (right), with red arrow pointing to the reaction chamber. (1) Buffer channel, (2) sample channel, (3) pressure channel, (4) passive valves (arrows), (5) mixer, (6) mixing channel, (7) washing chamber, (8) reaction chamber, and (9) waste chamber.
A schematic drawing of the laser welding setup.
Stereo microscope images from top direction of the welding seam using 3.15× and 64× zoom.
(a) For the temperature measurements, the cartridges were cut in half. The dashed red line represents the cutting line. (b) A schematic drawing of the test arrangement in the channel temperature measurements.
TSH-immunoassay in cartridges sealed either with an adhesive tape or using laser welding (2 × 4 scanning points), TSH concentration versus background-corrected—counts per second (cps).
The maximum channel temperatures during contour welding with 50 mm/s speed and 1.6 W laser power.
Thermal image captured 0.13 s after the beam has passed the line LI01 using a 100 μm welding distance between the seam and the channel wall.
Thermal image captured 3 s after the beam has passed the line LI01 using 100 μm welding distance between the seam and the channel wall. Temperature at the bottom of the channel is near to its maximum value.
Average fluorescence signals and coefficients of variance from model immunoassays for reaction chambers of the cartridges sealed either with an adhesive tape or by the use of laser welding.
Signal-to-background ratios for all TSH concentrations for the cartridges sealed with an adhesive tape and by use of laser welding.
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