Schematic overview of process flow in proposed microfluidic device.
Schematic illustration of proposed two-layer microfluidic chip. (Note that the top layer (glass-based) is used for DNA/enzyme reaction and the bottom layer (PMMA-based) is used for CE detection.)
Major steps in fabrication process used to realize proposed microfluidic chip.
Photograph of glass-based DNA reaction chip with a width and depth of 100 μm and 40 μm. (Note that the channel is filled with black dye for ease of visualization.)
Experimental setup used to evaluate performance of proposed microfluidic chip.
(a) Exploded representation of microfluidic device. (b) Experimental procedures and protocols for DNA digestion and CE analysis.
Experimental and numerical results for flow contours within T-form micromixer given different switching frequencies with a constant electric field intensity of 90 V/cm and a 200 MΩ resistor.
Evolution of mixing ratio in mixing channel given different switching frequencies with a constant electric field intensity of 90 V/cm and a 200 MΩ resistor.
Time-resolved electropherograms for digested φx-174 DNA sample (Note that the injection/separation voltage is 200 V/cm.)
Time-resolved electropherograms for φx-174 DNA sample given digestion times of: (a) 7 min, (b) 12 min, (c) 17 min, and (d) 22 min. (Note that the injection/separation voltage is 200 V/cm.)
Article metrics loading...
Full text loading...