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Integrated microfluidic chip for rapid DNA digestion and time-resolved capillary electrophoresis analysis
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10.1063/1.3654950
/content/aip/journal/bmf/6/1/10.1063/1.3654950
http://aip.metastore.ingenta.com/content/aip/journal/bmf/6/1/10.1063/1.3654950
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Schematic overview of process flow in proposed microfluidic device.

Image of FIG. 2.
FIG. 2.

Schematic illustration of proposed two-layer microfluidic chip. (Note that the top layer (glass-based) is used for DNA/enzyme reaction and the bottom layer (PMMA-based) is used for CE detection.)

Image of FIG. 3.
FIG. 3.

Major steps in fabrication process used to realize proposed microfluidic chip.

Image of FIG. 4.
FIG. 4.

Photograph of glass-based DNA reaction chip with a width and depth of 100 μm and 40 μm. (Note that the channel is filled with black dye for ease of visualization.)

Image of FIG. 5.
FIG. 5.

Experimental setup used to evaluate performance of proposed microfluidic chip.

Image of FIG. 6.
FIG. 6.

(a) Exploded representation of microfluidic device. (b) Experimental procedures and protocols for DNA digestion and CE analysis.

Image of FIG. 7.
FIG. 7.

Experimental and numerical results for flow contours within T-form micromixer given different switching frequencies with a constant electric field intensity of 90 V/cm and a 200 MΩ resistor.

Image of FIG. 8.
FIG. 8.

Evolution of mixing ratio in mixing channel given different switching frequencies with a constant electric field intensity of 90 V/cm and a 200 MΩ resistor.

Image of FIG. 9.
FIG. 9.

Time-resolved electropherograms for digested φx-174 DNA sample (Note that the injection/separation voltage is 200 V/cm.)

Image of FIG. 10.
FIG. 10.

Time-resolved electropherograms for φx-174 DNA sample given digestion times of: (a) 7 min, (b) 12 min, (c) 17 min, and (d) 22 min. (Note that the injection/separation voltage is 200 V/cm.)

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/content/aip/journal/bmf/6/1/10.1063/1.3654950
2012-03-15
2014-04-17
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Integrated microfluidic chip for rapid DNA digestion and time-resolved capillary electrophoresis analysis
http://aip.metastore.ingenta.com/content/aip/journal/bmf/6/1/10.1063/1.3654950
10.1063/1.3654950
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