1887
banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds
Rent:
Rent this article for
USD
10.1063/1.3671399
/content/aip/journal/bmf/6/1/10.1063/1.3671399
http://aip.metastore.ingenta.com/content/aip/journal/bmf/6/1/10.1063/1.3671399

Figures

Image of FIG. 1.
FIG. 1.

(a) PDMS-based micro-channel device with solution and gas has been pumped through the inlets and bubbles have been collected from the outlet. (b) Monodisperse bubbles with a uniform size were formed and constantly flowed out.

Image of FIG. 2.
FIG. 2.

Design of the fluidic chamber assembly.

Image of FIG. 3.
FIG. 3.

Side-view of the electrotaxis system.

Image of FIG. 4.
FIG. 4.

(a) Picture of a 3D scaffold. (b) Bright-field confocal image of a 3D scaffold. Only one fixed layer is shown. Scale bar = 50 μm. (c) Fluorescent confocal image of a 3D scaffold. Only one fixed layer is shown. Scale bar = 50 μm. (d) Re-constructed 3D top-view image of a 3D scaffold. (e) Re-constructed 3D side-view image of a 3D scaffold. (f) Re-constructed 3D image of CL1-0 cells (stained with CellVue®) inside a 3D scaffold (enhanced online) . [URL: http://dx.doi.org/10.1063/1.3671399.1]10.1063/1.3671399.1

Image of FIG. 5.
FIG. 5.

Pulmonary alveoli. An alveolus has a form of a hollow cavity, and in some alveolar walls, there are pores between alveoli called Pores of Kohn (reprinted from The President’s Council on Bioethics, Washington, D.C., January 2009, http://bioethics.georgetown.edu/pcbe/).

Image of FIG. 6.
FIG. 6.

Ellipticity of (a) CL1-0, (b) CL1-5, and (c) A549 cells after 2 h and 2 days cultured in 2D and 3D environments. (2D: 2D bare substrate, 2DG: 2D-gelatin coated substrate, 3DG: 3D-gelatin made scaffold.) For each cell line, the total number of cells selected for analysis is 30.

Image of FIG. 7.
FIG. 7.

A549 cells inside a 3D scaffold under an applied EF of 338 mV/mm at (a) t = 0 min and (b) t = 60 min. Clearly some cells (Nos. 1, 2, and 3 in blue circles) migrated through interconnected pores (enhanced online) . [URL: http://dx.doi.org/10.1063/1.3671399.2]10.1063/1.3671399.2

Image of FIG. 8.
FIG. 8.

Polar plots of cell migration after 2 h with and without the applied EF. Left column: CL1-0, CL1-5, and A549 without the applied EF. Right column: CL1-0, CL1-5, and A549 with the applied EF of 338 mV/mm. In the beginning, cells are set at the origin. All plots have the same scale and EF direction (from the right to the left).

Tables

Generic image for table
Table I.

Similarities between 3D porous scaffolds and in vivo pulmonary alveoli.

Generic image for table
Table II.

Migration directedness and speed of 3 different cells with and without the applied EF in 3D scaffolds. n is the total number of cells selected for analysis.

Generic image for table
Table III.

Electrotaxis of lung cancer cell lines CL1-0, CL1-5, and A549 in 2D and 3D environments. Stimulation time = 2 h (2D: 2D bare substrate, 2DG: 2D-gelatin coated substrate, 3D: 3D-gelatin made scaffold).

video/mp4,video/x-flv,video/flv,audio.mp3,audio.mpeg

Multimedia

The following multimedia file is available, if you log in: 1.3671399.original.v1.mov
The following multimedia file is available, if you log in: 1.3671399.original.v2.mov
Loading

Article metrics loading...

/content/aip/journal/bmf/6/1/10.1063/1.3671399
2012-01-04
2014-04-19
Loading

Full text loading...

This is a required field
Please enter a valid email address
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds
http://aip.metastore.ingenta.com/content/aip/journal/bmf/6/1/10.1063/1.3671399
10.1063/1.3671399
SEARCH_EXPAND_ITEM