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Cyclic olefin copolymer based microfluidic devices for biochip applications: Ultraviolet surface grafting using 2-methacryloyloxyethyl phosphorylcholine
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10.1063/1.3682098
/content/aip/journal/bmf/6/1/10.1063/1.3682098
http://aip.metastore.ingenta.com/content/aip/journal/bmf/6/1/10.1063/1.3682098

Figures

Image of FIG. 1.
FIG. 1.

Chemical structure of (a) COC, (b) MPC, and (c) schematic representation of the MPC grafting on COC, i.e., MPC solution containing benzophenone coated COC when exposed to UV light generate a free radical on the COC substrate by abstraction of H-atom from the substrate surface by the benzophenone followed by free radical polymerization of MPC.

Image of FIG. 2.
FIG. 2.

Effect of irradiation time on the extent of grafting on COC substrate [monomer concentration = 0.2 mol/L and initiator (BP) = concentration 0.2 mol/L].

Image of FIG. 3.
FIG. 3.

IR spectra of the original TOPAS substrate (unmodified) and MPC-grafted TOPAS substrates photografted using varying irradiation times [monomer concentration = 0.2 mol/L and BP concentration = 0.2 mol/L].

Image of FIG. 4.
FIG. 4.

Peak fitting of the C1s XPS spectra for (a) unmodified and (b) a typical MPC-grafted TOPAS substrate surface at a photoelectron takeoff angle of 90° [monomer concentration = 0.2 mol/L; initiator concentration = 0.2 mol/L; and irradiation time = 25 min].

Image of FIG. 5.
FIG. 5.

Effect of irradiation time on the surface contact angle of MPC modified TOPAS substrates [monomer concentration = 0.2 mol/L; initiator (BP) concentration = 0.2 mol/L].

Image of FIG. 6.
FIG. 6.

Fluorescence intensity of adsorbed fluorescein labeled BSA on unmodified and MPC grafted COC substrate. Average fluorescent intensity (n = 5) is plotted as a function of MPC grafting time [monomer concentration = 0.2 mol/L; initiator (BP) concentration = 0.2 mol/L].

Image of FIG. 7.
FIG. 7.

Cells attached on (a) unmodified COC substrate and (b) MPC grafted COC surface [monomer concentration = 0.2 mol/L; initiator (BP) concentration = 0.2 mol/L; and irradiation time = 25 min].

Image of FIG. 8.
FIG. 8.

(a) Comparison of cross-sectional channel profile of the grafted capillary electrophoresis microchip before and after thermal bonding [bonding temperature = 70 °C; bonding pressure = 2 MPa; and bonding time = 5 min]; (b) microchip that were filled with FITC-BSA solution indicating good sealing with no leakage as well as for protein adsorption measurement.

Tables

Generic image for table
Table I.

XPS analysis of elemental composition for untreated Topas and MPC grafted Topas substrates with varying irradiation time [monomer concentration 0.2 mol/L and initiator (BP) concentration 0.2 mol/L].

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/content/aip/journal/bmf/6/1/10.1063/1.3682098
2012-03-15
2014-04-16
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Cyclic olefin copolymer based microfluidic devices for biochip applications: Ultraviolet surface grafting using 2-methacryloyloxyethyl phosphorylcholine
http://aip.metastore.ingenta.com/content/aip/journal/bmf/6/1/10.1063/1.3682098
10.1063/1.3682098
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