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Rapid multi sample DNA amplification using rotary-linear polymerase chain reaction device (PCRDisc)
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10.1063/1.3690469
/content/aip/journal/bmf/6/1/10.1063/1.3690469
http://aip.metastore.ingenta.com/content/aip/journal/bmf/6/1/10.1063/1.3690469

Figures

Image of FIG. 1.
FIG. 1.

Schematic illustration of the PCRDisc and heaters.

Image of FIG. 2.
FIG. 2.

Rotary-linear motion system.

Image of FIG. 3.
FIG. 3.

Disc with sealed chamber.

Image of FIG. 4.
FIG. 4.

Schematic diagram of the 12 heater temperature control system.

Image of FIG. 5.
FIG. 5.

The completed PCRDisc system set-up ready for experimental procedures.

Image of FIG. 6.
FIG. 6.

Slab gel electrophoresis results for the inhibition and absorption experiment for the compact disc material. Lane M represents the 100bp ladder; lane 1, 2, and 3 represents the SPPS Internal control product with 150bp for the 3 PCR chambers.

Image of FIG. 7.
FIG. 7.

Slab gel electrophoresis results for different heating duration at each temperature zone. Lane M, C, 1, 2, and 3 represent the 100bp ladder, control, 10 s heating, 15 s heating, and 20 s heating, respectively.

Image of FIG. 8.
FIG. 8.

Amplification results of four samples using the PCRDisc. Lane M and C correspond to the 100bp ladder and control, respectively. Lane 1, 2, 3, and 4 represents the amplified samples from the disc.

Image of FIG. 9.
FIG. 9.

Amplification of eight samples using the PCRDisc. Lane M and C correspond to the 100bp ladder and control, respectively. Lane 1-8 represents the amplified samples from the disc.

Image of FIG. 10.
FIG. 10.

Twelve sample amplification using PCRDisc. Lane M and C correspond to the 100bp ladder and control, respectively. Lane 1-12 represents the amplified samples from the disc.

Image of FIG. 11.
FIG. 11.

(a) Simplified cross sectional view of a single chamber of the compact disc PCR chip. (b) Location of the temperature probes in the sample chamber and boundary conditions.

Image of FIG. 12.
FIG. 12.

PCRDisc temperature cycling using 15 s heating duration.

Tables

Generic image for table
Table I.

Material physical properties.

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/content/aip/journal/bmf/6/1/10.1063/1.3690469
2012-03-14
2014-04-17
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Rapid multi sample DNA amplification using rotary-linear polymerase chain reaction device (PCRDisc)
http://aip.metastore.ingenta.com/content/aip/journal/bmf/6/1/10.1063/1.3690469
10.1063/1.3690469
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