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An integrated, multiparametric flow cytometry chip using “microfluidic drifting” based three-dimensional hydrodynamic focusing
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10.1063/1.3701566
/content/aip/journal/bmf/6/2/10.1063/1.3701566
http://aip.metastore.ingenta.com/content/aip/journal/bmf/6/2/10.1063/1.3701566

Figures

Image of FIG. 1.
FIG. 1.

Configuration of the flow cytometry chip. The fluidic channel is for 3D on-chip focusing of particles. Inlets A and B are for particles and the vertical-focusing sheath flow, respectively. Inlets C and D are for the horizontal-focusing sheath flow. The arrangement of the optical fibers is indicated in the figure.

Image of FIG. 2.
FIG. 2.

(a) An assembled flow cytometry chip whose size is comparable to a U.S. quarter. The fluidic channel, optical fibers, and coupled laser beam can be clearly seen in the image. (b) A microscopic image indicating hydrodynamic focusing and the arrangement of the optical fibers. Inlet A is mixed with fluorescent dye to show the focused stream.

Image of FIG. 3.
FIG. 3.

Fabrication procedure of the flow cytometry chip. (a) PDMS layer for fluidic channel/fiber-insertion channel. (b) PDMS layer sealed with a glass substrate. (c) Insertion of fluidic tubings. (d) Insertion of optical fibers.

Image of FIG. 4.
FIG. 4.

Simultaneous detection of FSC, SSC, and FL signals from two types of fluorescent microparticles. The inset shows a 10 ms snapshot.

Image of FIG. 5.
FIG. 5.

Comparison of 3D scatter plots obtained from (a) the flow cytometry chip and (b) a commercial flow cytometer (Beckman-Coulter FC500).

Tables

Generic image for table
Table I.

Comparison of CVs of the flow cytometry chip and a commercial flow cytometer (Beckman-Coulter FC500) for the same sets of fluorescent microparticles (particles #1 and #2).

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/content/aip/journal/bmf/6/2/10.1063/1.3701566
2012-04-20
2014-04-25
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: An integrated, multiparametric flow cytometry chip using “microfluidic drifting” based three-dimensional hydrodynamic focusing
http://aip.metastore.ingenta.com/content/aip/journal/bmf/6/2/10.1063/1.3701566
10.1063/1.3701566
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