Configuration of the flow cytometry chip. The fluidic channel is for 3D on-chip focusing of particles. Inlets A and B are for particles and the vertical-focusing sheath flow, respectively. Inlets C and D are for the horizontal-focusing sheath flow. The arrangement of the optical fibers is indicated in the figure.
(a) An assembled flow cytometry chip whose size is comparable to a U.S. quarter. The fluidic channel, optical fibers, and coupled laser beam can be clearly seen in the image. (b) A microscopic image indicating hydrodynamic focusing and the arrangement of the optical fibers. Inlet A is mixed with fluorescent dye to show the focused stream.
Fabrication procedure of the flow cytometry chip. (a) PDMS layer for fluidic channel/fiber-insertion channel. (b) PDMS layer sealed with a glass substrate. (c) Insertion of fluidic tubings. (d) Insertion of optical fibers.
Simultaneous detection of FSC, SSC, and FL signals from two types of fluorescent microparticles. The inset shows a 10 ms snapshot.
Comparison of 3D scatter plots obtained from (a) the flow cytometry chip and (b) a commercial flow cytometer (Beckman-Coulter FC500).
Comparison of CVs of the flow cytometry chip and a commercial flow cytometer (Beckman-Coulter FC500) for the same sets of fluorescent microparticles (particles #1 and #2).
Article metrics loading...
Full text loading...