Flow cytometry plots of ES and DE cells' expression of the carbohydrates recognizing the lectins DBA, PNA, and UEAI. The shaded population represents the negative control, while unshaded population represents the cells binding to the ligand. Both cell types express high levels of carbohydrate patterns for the lectins studied.
Flow cytometry plots for ES and DE cells expression of common stem cell markers. The shaded population represents the negative control, while unshaded population represents the cells binding to the ligand. DE and ES cells have different expressions of SSEA3 and CXCR4.
Cell adhesion as a function of shear stress with (a) human embryonic stem cells and (b) Definitive endodermal cells on ligand coated surfaces. DE cells in general have a higher affinity for each ligand than ES cells with the exception of UEAI that ES cells have a substantially high affinity for.
(a) Comparison of ES and DE cell adhesion to a panel of adhesive molecules at a shear stress of 0.74 dyn/cm2. *denotes significant difference with p = 0.009 and ** denotes significant difference with p < 0.003. (b) Evaluation of lectin and stem cell maker expression for ES and DE cell populations with flow cytometry. Microfluidic and flow cytometry differ for some ligand studied.
The kinetics of DE and ES cells adhesion to DBA and anti-SSEA3 coated parallel flow devices. These data points are fitted to a pseudo-first order kinetic model (Eq. (4)). Longer incubation time results in less cell detachment.
Acronyms used with their respective definitions.
Fitted parameters to the cell binding kinetic model for the varying cell ligand interactions.
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