1887
banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
Lectin-functionalized microchannels for characterizing pluripotent cells and early differentiation
Rent:
Rent this article for
USD
10.1063/1.4719979
/content/aip/journal/bmf/6/2/10.1063/1.4719979
http://aip.metastore.ingenta.com/content/aip/journal/bmf/6/2/10.1063/1.4719979

Figures

Image of FIG. 1.
FIG. 1.

Flow cytometry plots of ES and DE cells' expression of the carbohydrates recognizing the lectins DBA, PNA, and UEAI. The shaded population represents the negative control, while unshaded population represents the cells binding to the ligand. Both cell types express high levels of carbohydrate patterns for the lectins studied.

Image of FIG. 2.
FIG. 2.

Flow cytometry plots for ES and DE cells expression of common stem cell markers. The shaded population represents the negative control, while unshaded population represents the cells binding to the ligand. DE and ES cells have different expressions of SSEA3 and CXCR4.

Image of FIG. 3.
FIG. 3.

Cell adhesion as a function of shear stress with (a) human embryonic stem cells and (b) Definitive endodermal cells on ligand coated surfaces. DE cells in general have a higher affinity for each ligand than ES cells with the exception of UEAI that ES cells have a substantially high affinity for.

Image of FIG. 4.
FIG. 4.

(a) Comparison of ES and DE cell adhesion to a panel of adhesive molecules at a shear stress of 0.74 dyn/cm2. *denotes significant difference with p = 0.009 and ** denotes significant difference with p < 0.003. (b) Evaluation of lectin and stem cell maker expression for ES and DE cell populations with flow cytometry. Microfluidic and flow cytometry differ for some ligand studied.

Image of FIG. 5.
FIG. 5.

The kinetics of DE and ES cells adhesion to DBA and anti-SSEA3 coated parallel flow devices. These data points are fitted to a pseudo-first order kinetic model (Eq. (4)). Longer incubation time results in less cell detachment.

Tables

Generic image for table
Table I.

Acronyms used with their respective definitions.

Generic image for table
Table II.

Fitted parameters to the cell binding kinetic model for the varying cell ligand interactions.

Loading

Article metrics loading...

/content/aip/journal/bmf/6/2/10.1063/1.4719979
2012-05-22
2014-04-24
Loading

Full text loading...

This is a required field
Please enter a valid email address
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Lectin-functionalized microchannels for characterizing pluripotent cells and early differentiation
http://aip.metastore.ingenta.com/content/aip/journal/bmf/6/2/10.1063/1.4719979
10.1063/1.4719979
SEARCH_EXPAND_ITEM