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On the influence of the architecture of poly(ethylene glycol)-based thermoresponsive polymers on cell adhesion
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View: Figures


Image of FIG. 1.
FIG. 1.

Chemical structure of the three poly(MEO2MA-co-OEGMA)-based TRP which differs in their ratio of MEO2MA (x) and OEGMA (y) and the chain length (z) of the OEGMA side chain. The table also gives the resulting LCST measured by turbidimetry and the molecular weight determined by gel permeation chromatography.

Image of FIG. 2.
FIG. 2.

Phase contrast images of L929 mouse fibroblasts on different poly(MEO2MA-co-OEGMA)-coated gold substrates and uncoated gold substrates after different cultivation durations. The time needed for complete cell spreading after seeding and subsequent sedimentation of spherical cells from suspension strongly depends on the polymer. The concentration of the coating solution was 5 μM in each case. The scale bar is 100 μm.

Image of FIG. 3.
FIG. 3.

Spreading time (a) and rounding efficiency (b) of L929 mouse fibroblasts on substrates coated with different poly(MEO2MA-co-OEGMA) polymers as a function of the polymer concentration of the coating solution. The lines only serve as guides to the eye.

Image of FIG. 4.
FIG. 4.

Optical phase contrast images of L929 mouse fibroblasts on TRP-coated gold substrates (a)–(c) and on pure gold substrates (d)–(f) were taken at 37 °C (a) and (d) and after being exposed to room temperature (∼22 °C) for 30 min (b) and (e). After this treatment, the cells could easily be removed by rinsing (c) and (f). The scale bars are 100 μm.

Image of FIG. 5.
FIG. 5.

(a) Measurement of the surface plasmon resonance on a blank gold substrate (black) and after immobilization of 25 μM polymer I (gray). The shift of the resonance angle Δθ of 0.8° corresponds to a layer thickness of 4.5 nm using a refractive index of 1.5. (b) Kinetic immobilization measurements of polymer I on a gold substrate at a constant angle (55.5°). After 2 h, the reflectivity signal goes into saturation. For details see text.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: On the influence of the architecture of poly(ethylene glycol)-based thermoresponsive polymers on cell adhesion