1887
banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
Fabrication of long poly(dimethyl siloxane) nanochannels by replicating protein deposit from confined solution evaporation
Rent:
Rent this article for
USD
10.1063/1.4730371
/content/aip/journal/bmf/6/2/10.1063/1.4730371
http://aip.metastore.ingenta.com/content/aip/journal/bmf/6/2/10.1063/1.4730371
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

The schematic of confined evaporation process for fabrication of PDMS nanochannel array. The protein (i.e., BSA) solution evaporates and the BSA molecules deposit inside microchannels to form line structures at nano/micro scale. PDMS nanochannels can be fabricated by replicating the line structures, followed by bonding the micro/nanogrooves onto glass substrate.

Image of FIG. 2.
FIG. 2.

The images of BSA deposit for solution concentration (a) 0.5 wt. % and (b) 1 wt. %. (a1) and (b1) are the optical images. (a2) and (b2) are SEM images. (a3) and (b3) are AFM images with corresponding measurements (a4) and (b4). (The scale bar in the inset of SEM images is 100 nm.)

Image of FIG. 3.
FIG. 3.

The SEM images of BSA deposit at the bottom corners of and along the microchannel.

Image of FIG. 4.
FIG. 4.

The height of the deposited BSA structures at different solution concentrations. The average heights of the deposited BSA structures from using 0.5 wt. %, 1 wt. %, 2 wt. %, and 3 wt. % solution are approximately 210, 530, 1030, and 1840 nm, respectively.

Image of FIG. 5.
FIG. 5.

The images of (a) the deposited BSA structures and (b) the negative relief near the open end of the microchannels (1 wt. % solution was used)

Image of FIG. 6.
FIG. 6.

(a) Top view and (b) 45° tilted view of the negative relief of the deposited BSA structures. (The scale bar in the inset is 1 μm)

Image of FIG. 7.
FIG. 7.

The nanochannels are filled with the 0.1 wt. % BSA solution from the inlet to the outlet. The solution was added with fluorescein dye for visualization. (“L” stands for the distance from the inlet)

Image of FIG. 8.
FIG. 8.

Stretching of DNA molecules (a) near the inlet (micro to nano region) and (b) at about 2 mm away from the inlet of the nanochannel. The maximum elongation of λ-DNA reaches 10 μm, which is about 50% of its fully extended length. (Scale bar: 20 μm)

Loading

Article metrics loading...

/content/aip/journal/bmf/6/2/10.1063/1.4730371
2012-06-19
2014-04-25
Loading

Full text loading...

This is a required field
Please enter a valid email address
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Fabrication of long poly(dimethyl siloxane) nanochannels by replicating protein deposit from confined solution evaporation
http://aip.metastore.ingenta.com/content/aip/journal/bmf/6/2/10.1063/1.4730371
10.1063/1.4730371
SEARCH_EXPAND_ITEM