Schematic diagram of the ApoStream device; inset shows cell flow and separation in the flow chamber. (For details, see explanation in the text).
(a) Schematic of the top view of the flow chamber showing sample injection and sample collection port locations. (b) Still image from video demonstrating the flow and collection of fluorescently labeled SKOV3 cancer cells through the collection port in the ApoStream flow chamber. Cancer cells are collected into the collection port when the DEP field is activated. (c) Still image from video demonstrating the flow of fluorescently labeled PBMCs through ApoStream flow chamber. The first half of the video (∼10 s) demonstrates that most PBMCs fall into the collection port when the DEP field is not active. The second half of the video (from 11 to 21 s) demonstrates that upon the activation of DEP field the PBMCs are repelled from the electrode causing them to move into the high velocity flow region and are no longer being pulled into the collection port (enhanced online). [URL: http://dx.doi.org/10.1063/1.4731647.1] [URL: http://dx.doi.org/10.1063/1.4731647.2]10.1063/1.4731647.110.1063/1.4731647.2
(a) RSM plot for the ApoStream flow chamber showing cancer cell recovery response to frequency and sample injection flow rate. (b) Effect of operating frequency on percentage PBMC reduction after ApoStream separation.
Buffer optimization for the ApoStream device using SKOV3 cancer cells spiked in PBMCs. (a) Pareto chart from DOE analysis showing sample buffer composition as the only significant factor with a positive effect on cancer cell recovery. (b) Sample buffer with additives improves cancer cell recovery.
ApoStream device linearity for (a) SKOV3 and (b) MDA-MB-231 spiked cell recovery.
Images of cultured MDA-MB-231 cancer cells at day 2 and day 7: (a) and (b) control cells (no ApoStream separation); (c) and (d) cells captured with ApoStream; (e) ApoStream recovered MDA-MB-231 cancer cells show exponential growth and no difference compared to control cells (no ApoStream separation).
Intra-day and inter-day precision of the ApoStream device for the recovery of approximately 5000 SKOV3 and 500 MDA-MB-231 cells spiked into PBMCs from 7.5 ml of normal human donor blood.
Cancer cell recovery from ApoStream device for low number of cancer cells spiked into PBMCs from 7.5 ml of normal human donor blood.
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