No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.
Dielectrophoresis has broad applicability to marker-free isolation of tumor cells from blood by microfluidic systems
1. S. Dawood, K. Broglio, V. Valero, J. Reuben, B. Handy, R. Islam, S. Jackson, G. N. Hortobagyi, H. Fritsche, and M. Cristofanilli, Cancer 113, 2422–2430 (2008).
2. J. S. de Bono, H. I. Scher, R. B. Montgomery, C. Parker, M. C. Miller, H. Tissing, G. V. Doyle, L. W. Terstappen, K. J. Pienta, and D. Raghavan, Clin. Cancer Res. 14, 6302–6309 (2008).
3. N. J. Meropol, Clin. Adv. Hematol. Oncol. 7, 247–248 (2009).
4. W. Xu, L. Cao, L. Chen, J. Li, X. F. Zhang, H. H. Qian, X. Y. Kang, Y. Zhang, J. Liao, L. H. Shi, Y. F. Yang, M. C. Wu, and Z. F. Yin, Clin. Cancer Res. 17, 3783–3793 (2011).
5. K. Koyanagi, S. J. O'Day, P. Boasberg, M. B. Atkins, H. J. Wang, R. Gonzalez, K. Lewis, J. A. Thompson, C. M. Anderson, J. Lutzky, T. T. Amatruda, E. Hersh, J. Richards, J. S. Weber, and D. S. Hoon, Clin. Cancer Res. 16, 2402–2408 (2010).
6. T. Kurihara, T. Itoi, A. Sofuni, F. Itokawa, T. Tsuchiya, S. Tsuji, K. Ishii, N. Ikeuchi, A. Tsuchida, K. Kasuya, T. Kawai, Y. Sakai, and F. Moriyasu, J. Hepatobiliary Pancreat Surg. 15, 189–195 (2008).
7. N. Bednarz-Knoll, C. Alix-Panabieres, and K. Pantel, Breast Cancer Res. 13, 228 (2011).
9. M. A. Leversha, J. Han, Z. Asgari, D. C. Danila, O. Lin, R. Gonzalez-Espinoza, A. Anand, H. Lilja, G. Heller, M. Fleisher, and H. I. Scher, Clin. Cancer Res. 15, 2091–2097 (2009).
10. K. Pachmann, O. Camara, T. Kroll, M. Gajda, A. K. Gellner, J. Wotschadlo, and I. B. Runnebaum, J. Cancer Res. Clin. Oncol. 137, 1317–1327 (2011).
11. M. C. Miller, G. V. Doyle, and L. W. Terstappen, J. Oncol. 2010, 617421.
12. S. J. Cohen, C. J. Punt, N. Iannotti, B. H. Saidman, K. D. Sabbath, N. Y. Gabrail, J. Picus, M. Morse, E. Mitchell, M. C. Miller, G. V. Doyle, H. Tissing, L. W. Terstappen, and N. J. Meropol, J. Clin. Oncol. 26, 3213–3221 (2008).
13. C. Raimondi, A. Gradilone, G. Naso, B. Vincenzi, A. Petracca, C. Nicolazzo, A. Palazzo, R. Saltarelli, F. Spremberg, E. Cortesi, and P. Gazzaniga, Breast Cancer Res. Treat 130, 449–455 (2011).
14. A. Bonnomet, A. Brysse, A. Tachsidis, M. Waltham, E. W. Thompson, M. Polette, and C. Gilles, J. Mammary Gland Biol. Neoplasia 15, 261–273 (2010).
25. U. T. Shankavaram, W. C. Reinhold, S. Nishizuka, S. Major, D. Morita, K. K. Chary, M. A. Reimers, U. Scherf, A. Kahn, D. Dolginow, J. Cossman, E. P. Kaldjian, D. A. Scudiero, E. Petricoin, L. Liotta, J. K. Lee, and J. N. Weinstein, Mol. Cancer Ther. 6, 820–832 (2007).
26. A. V. Roschke, G. Tonon, K. S. Gehlhaus, N. McTyre, K. J. Bussey, S. Lababidi, D. A. Scudiero, J. N. Weinstein, and I. R. Kirsch, Cancer Res. 63, 8634–8647 (2003).
27. P. R. C. Gascoyne, “Isolation and characterization of cells by dielectrophoretic field-flow fractionation,” in Field-Flow Fractionation in Biopolymer Analysis (Springer-Verlag, 2012).
41. P. R. C. Gascoyne, S. Shim, J. Noshari, F. F. Becker, H. Huang, R. Pethig, J. Vykoukal, P. R. C. Gascoyne, and K. Stemke-Hale, “Correlations between the dielectric properties and exterior morphology of cells revealed by dielectrophoretic field-flow fractionation,” Electrophoresis (in press).
44. M. Cristofanilli, G. De Gasperis, L. Zhang, M.-C. Hung, P. R. C. Gascoyne, and G. N. Hortobagyi, Clin. Cancer Res. 8, 615–619 (2002).
52. R. Rosenberg, R. Gertler, J. Friederichs, K. Fuehrer, M. Dahm, R. Phelps, S. Thorban, H. Nekarda, and J. R. Siewert, Cytometry 49, 150–158 (2002).
Article metrics loading...
The number of circulating tumor cells (CTCs) found in blood is known to be a prognostic marker for recurrence of primary tumors, however, most current methods for isolating CTCs rely on cell surface markers that are not universally expressed by CTCs. Dielectrophoresis (DEP) can discriminate and manipulate cancer cells in microfluidic systems and has been proposed as a molecular marker-independent approach for isolating CTCs from blood. To investigate the potential applicability of DEP to different cancer types, the dielectric and density properties of the NCI-60 panel of tumor cell types have been measured by dielectrophoretic field-flow fractionation (DEP-FFF) and compared with like properties of the subpopulations of normal peripheral blood cells. We show that all of the NCI-60 cell types, regardless of tissue of origin, exhibit dielectric properties that facilitate their isolation from blood by DEP. Cell types derived from solid tumors that grew in adherent cultures exhibited dielectric properties that were strikingly different from those of peripheral blood cell subpopulations while leukemia-derived lines that grew in non-adherent cultures exhibited dielectric properties that were closer to those of peripheral blood cell types. Our results suggest that DEP methods have wide applicability for the surface-marker independent isolation of viable CTCs from blood as well as for the concentration of leukemia cells from blood.
Full text loading...
Most read this month