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Size-based hydrodynamic rare tumor cell separation in curved microfluidic channels
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10.1063/1.4774311
/content/aip/journal/bmf/7/1/10.1063/1.4774311
http://aip.metastore.ingenta.com/content/aip/journal/bmf/7/1/10.1063/1.4774311

Figures

Image of FIG. 1.
FIG. 1.

(a) Schematics of the microfluidic cell sorter containing 6-loop double spiral microchannel for particle/cell separation. (b) Picture of the assembled cell sorter.

Image of FIG. 2.
FIG. 2.

Comparison of the Dean vortices and their magnitude at (a) 10 ml/h, and (b) 60 ml/h at the S-shape junction. The small arrows represent the velocity vector projected onto the cross-section and color contours represent the magnitude of the Dean flow velocity. The big red arrow indicates the flow direction.

Image of FIG. 3.
FIG. 3.

Simulating prediction of trajectories of two types of particles near the inner/middle/outer outlet of the double spiral microchannel. (a) Flow rate 10 ml/h; (b) flow rate 20 ml/h; (c) flow rate 30 ml/h; (d) flow rate 40 ml/h; (e) flow rate 50 ml/h; (f) flow rate 60 ml/h. The red and blue dashed lines denote 5 μm particles and 15 μm particles, respectively.

Image of FIG. 4.
FIG. 4.

Numerical prediction of the trajectories of 5 - and 15 -μm particles along the double spiral channel at the flow rate of 60 ml/h. The red and blue dashed lines denote 5 μm particles and 15 μm particles, respectively. The arrow represents the flow direction.

Image of FIG. 5.
FIG. 5.

(a)–(f) Superimposed fluorescent images at the center of the double spiral channel for 5 μm (red line) and 15 μm (green line) particles at six different flow rates ranging from 10 to 60 ml/h. The arrow indicates the flow direction. (g) Line scans across the 300 μm wide channel of each composite image taken at the center to illustrate the distribution and position of 5 μm and 15 μm particles. (a′)–(f′) Superimposed fluorescent images at the outlet of the double spiral channel for 5 μm (red line) and 15 μm (green line) particles at the same flow rates. (g′) Line scans across the 300 μm wide channel of each composite image taken at the outlet. (a″)–(f″) Superimposed fluorescent images at the outlet of the single spiral channel. (g″) Line scans across the outlet of the single spiral channel.

Image of FIG. 6.
FIG. 6.

(a) The optical microscope image indicating the trajectories of blood cells, (b) the fluorescent microscope image of HeLa cells, and (c) the superimposed image at the outlet of the double spiral channel at 40 ml/h. HeLa cells are stained with green and 20 × diluted blood cells are unstained. (a′)–(c′) The optical, fluorescent and superimposed microscope images taken at the outlet of the double spiral channel at 60 ml/h. (d) The composite image of cells collected at the middle outlet of the double spiral at 60 ml/h. (e) The composite image of cells collected at the inner outlet at 60 ml/h.

Tables

Generic image for table
Table I.

The statistical results of particle purity in the inner and the middle outlets of the double/single spiral microchannel for separating binary mixture of 5- and 15-μm particles.

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/content/aip/journal/bmf/7/1/10.1063/1.4774311
2013-01-07
2014-04-19
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Size-based hydrodynamic rare tumor cell separation in curved microfluidic channels
http://aip.metastore.ingenta.com/content/aip/journal/bmf/7/1/10.1063/1.4774311
10.1063/1.4774311
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