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Size-based hydrodynamic rare tumor cell separation in curved microfluidic channels
1. R. T. Krivacic, A. Ladanyi, D. N. Curry, H. B. Hsieh, P. Kuhn, D. E. Bergsrud, J. F. Kepros, T. Barbera, M. Y. Ho, L. B. Chen, R. A. Lerner, and R. H. Bruce, Proc. Natl. Acad. Sci. U.S.A. 101, 10501 (2004).
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In this work, we propose a rapid and continuous rare tumor cell separation based on hydrodynamic effects in a label-free manner. The competition between the inertial lift force and Dean drag force inside a double spiral microchannel results in the size-based cell separation of large tumor cells and small blood cells. The mechanism of hydrodynamic separation in curved microchannel was investigated by a numerical model. Experiments with binary mixture of 5- and 15-μm-diameter polystyrene particles using the double spiral channel showed a separation purity of more than 95% at the flow rate above 30 ml/h. High throughput (2.5 × 108 cells/min) and efficient cell separation (more than 90%) of spiked HeLa cells and 20 × diluted blood cells was also achieved by the double spiral channel.
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