Schematic view of the multi-stage multi-orifice flow fractionation (MS-MOFF) system. At the first MOFF stage, the RBCs and WBCs split into two positions, and the MCF-7 cells focused at the middle of the channel (inside). At the second MOFF stage, RBCs and WBCs directed to the side channel (outside), similar to the first stage. On the other hand, a few MCF-7 cells that travelled through the side channel at the first stage (non-selected target) were directed to the middle of the channel (inside).
Photographic image of the MS-MOFF fabricated device.
Trajectory of cells through the multi-orifice microchannel according to channel Reynolds number (Rec ). A picture of the first stage separation region; two small pictures of the upper and lower second stage separation regions. When Rec was 70 at the first stage MOFF, the best separation of MCF-7 and blood cells was achieved at this flow rate condition.
Trajectory of MCF-7 cells through the multi-orifice microchannel according to Qc/Qm condition. Qc/Qm values represent the used portion of the central collecting channel. Qc, flow rate of the central collecting channel in the first MOFF stage; Qm, total flow rate in the first MOFF stage.
Size distribution of MCF-7 cells used in this study. The histogram was derived using an automated cell counter (Scepter, Millipore Co.). The coefficient of variation was relatively large (19.12%) since cell size depends on various conditions such as cell cycle and the microenvironment.
Flow rate (μl/min) of each inlet and outlet at various Qc/Qm conditions.
Separation efficiencies of blood and MCF-7 cells in the MS-MOFF depending on the Qc/Qm condition. The inside fraction exits to outlet 1 and the outside fraction to outlets 2 through 5.
Separation efficiency in comparison of SS-MOFF, MOFF+DEP and MS-MOFF.
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