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Protein sensing by nanofluidic crystal and its signal enhancement
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Image of FIG. 1.
FIG. 1.

Schematic illustrations of the present nanofluidic crystal based protein sensor and its signal enhancement. (a) Sensing principle of the present nanofluidic crystal; (b) equivalent electrical model of the sensing unit; (c) basic surface status, thrombin captured status, and signal enhancement status, from top to bottom; (d) corresponding I-V curves for three different surface statuses in (c).

Image of FIG. 2.
FIG. 2.

The experimental setup and the chip. (a) The whole experimental system; (b) Photo of the micropore chip with a penny for comparison. Inserted is a SEM photo of the packed nanoparticles (from the pore-side). The scale bar is 5 μm.

Image of FIG. 3.
FIG. 3.

Variation of conductance with time during the stabilization of nanofluidic crystal under 10 V bias.

Image of FIG. 4.
FIG. 4.

Variation of the ionic current with time during electrophoresis loading of thrombin molecules. The current keeps stable in DI water without loading thrombin.

Image of FIG. 5.
FIG. 5.

Conductance variation with thrombin concentration in DI water. The inserted photo shows a typical I-V curve. The error bars represent three independent tests.

Image of FIG. 6.
FIG. 6.

Comparison of the conductance variations in the experiment group and three control groups.

Image of FIG. 7.
FIG. 7.

Variation of the measured surface Zeta potentials (absolute values, all measured Zeta potentials were negative) and the calculated surface charge densities with the thrombin concentration. The columns (data on top) represented the ratios of the calculated surface charge density to the measured Zeta potential in each case, which theoretically should be the same.


Generic image for table
Table I.

Sequences of oligonucleotides (DNA/aptamers) used in the present work.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Protein sensing by nanofluidic crystal and its signal enhancement