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A microfluidic chip for direct and rapid trapping of white blood cells from whole blood
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10.1063/1.4808179
/content/aip/journal/bmf/7/3/10.1063/1.4808179
http://aip.metastore.ingenta.com/content/aip/journal/bmf/7/3/10.1063/1.4808179
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Fabrication process of the chip. (a) A Si wafer; (b) sputtering a Cr/Cu seed layer; (c) electroforming Cu; (d) electroforming Cu; (e) transferring the structure to PDMS; and (f) punching inlets/outlet and bonding PDMS and a glass slide.

Image of FIG. 2.
FIG. 2.

Experimental setup for tartrazine and brilliant blue water mixing.

Image of FIG. 3.
FIG. 3.

(a) Photographs of the chip; (b) SEM photo of the winding channel, and (c) SEM photo of the trapping structure.

Image of FIG. 4.
FIG. 4.

Tartrazine and brilliant blue water mixing under different flow velocities (blue ring is the starting of complete mixing). (a) 0.5 l/min; (b) 0.25 l/min; (c) 0.125 l/min; and (d) 0.1 l/min.

Image of FIG. 5.
FIG. 5.

WBCs trapping under the flow velocity 0.25 l/min.

Image of FIG. 6.
FIG. 6.

(a) Lysis efficiency of red blood cells in the microfluidic channel and (b) WBCs trapping under the flow velocity 0.05 l/min.

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/content/aip/journal/bmf/7/3/10.1063/1.4808179
2013-06-03
2014-04-24
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: A microfluidic chip for direct and rapid trapping of white blood cells from whole blood
http://aip.metastore.ingenta.com/content/aip/journal/bmf/7/3/10.1063/1.4808179
10.1063/1.4808179
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