Fabrication process of the chip. (a) A Si wafer; (b) sputtering a Cr/Cu seed layer; (c) electroforming Cu; (d) electroforming Cu; (e) transferring the structure to PDMS; and (f) punching inlets/outlet and bonding PDMS and a glass slide.
Experimental setup for tartrazine and brilliant blue water mixing.
(a) Photographs of the chip; (b) SEM photo of the winding channel, and (c) SEM photo of the trapping structure.
Tartrazine and brilliant blue water mixing under different flow velocities (blue ring is the starting of complete mixing). (a) 0.5 μl/min; (b) 0.25 μl/min; (c) 0.125 μl/min; and (d) 0.1 μl/min.
WBCs trapping under the flow velocity 0.25 μl/min.
(a) Lysis efficiency of red blood cells in the microfluidic channel and (b) WBCs trapping under the flow velocity 0.05 μl/min.
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