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Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays
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10.1063/1.4811278
/content/aip/journal/bmf/7/3/10.1063/1.4811278
http://aip.metastore.ingenta.com/content/aip/journal/bmf/7/3/10.1063/1.4811278
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Schematic illustration of the microfluidic framework with built-in function patterns (in red), inspired by 2D barcodes. Two structures are proposed. Structure 1 is a semi-open frame structure, similar to the Data Matrix scheme. Structure 2 is a closed-frame structure, similar to the QR code scheme. F1, F2, F3, F1′, F3′: function pattern. , , : characteristic angle, spacing, and width of the function patterns. α, , : characteristic angle, spacing, and width of the array spots.

Image of FIG. 2.
FIG. 2.

Layout of the 2D microfluidic chip.

Image of FIG. 3.
FIG. 3.

Flow chart of the program.

Image of FIG. 4.
FIG. 4.

The high throughput immunoassay using the semi-opened function pattern-incorporated microfluidic network (structure 1). (a) The scheme for sample introduction. This is a concentration gradient experiment. We highlight the function patterns in red. (b)–(e) We captured the fluorescence images in different styles. (f)–(i) The 3D plots of the arithmetic mean for (b)–(e), respectively. (j)–(m) The 3D plots of the median for (b)–(e), respectively. The white asterisks in (f)–(m) indicate the origin of the 3D plots and correspond to the white asterisks in (b)–(e), respectively. This origin is shown in (a) in a black square.

Image of FIG. 5.
FIG. 5.

The high throughput immunoassay using the closed function pattern-incorporated microfluidic network (structure 2). (a) The scheme for sample introduction. This is a concentration gradient experiment. We highlight the function patterns in red. (b)–(d) We captured the fluorescence images in different styles. (e)–(g) The 3D plots of the arithmetic mean for (b)–(d), respectively. (h)–(j) The 3D plots of the median for (b)–(d), respectively. The white asterisks in (e)–(j) indicate the origin of the 3D plots and correspond to the white asterisks in (b)–(d), respectively. This origin is shown in (a) in a black square.

Image of FIG. 6.
FIG. 6.

The generalized approach for the construction of function patterns. The function patterns and the corresponding molecules used in the construction are highlighted in red.

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/content/aip/journal/bmf/7/3/10.1063/1.4811278
2013-06-13
2014-04-18
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays
http://aip.metastore.ingenta.com/content/aip/journal/bmf/7/3/10.1063/1.4811278
10.1063/1.4811278
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