In this paper, we present a low cost and equipment-free blood filtration device capable of producing plasma from blood samples with mL-scale capacity and demonstrate its clinical application for hepatitis B diagnosis. We report the results of in-field testing of the device with 0.8–1 ml of undiluted, anticoagulated human whole blood samples from patients at the National Hospital for Tropical Diseases in Hanoi, Vietnam. Blood cell counts demonstrate that the device is capable of filtering out 99.9% of red and 96.9% of white blood cells, and the plasma collected from the device contains lower red blood cell counts than plasma obtained from a centrifuge. Biochemistry and immunology testing establish the suitability of the device as a sample preparation unit for testing alanine transaminase (ALT), aspartate transaminase (AST), urea, hepatitis B “e” antigen (HBeAg), hepatitis B “e” antibody (HBe Ab), and hepatitis B surface antibody (HBs Ab). The device provides a simple and practical front-end sample processing method for point-of-care microfluidic diagnostics, enabling sufficient volumes for multiplexed downstream tests.
The authors gratefully acknowledge financial support from Grand Challenges Canada (Grant No. 0005-02-02-01-01). We would also like to acknowledge the assistance and support of the staff at the National Hospital for Tropical Diseases in Hanoi, Vietnam. Most notably, testing by Dr. Van Dinh Trang is gratefully acknowledged. The authors also gratefully acknowledge infrastructure funding from the Canada Foundation for Innovation (CFI) and on-going support from the Natural Sciences and Engineering Research Council of Canada (NSERC).
A. Device design and fabrication
B. Blood cell count, biochemistry, and immunology testing
III. RESULTS AND DISCUSSION
A. Volume of filtered plasma
B. Blood cell count results
C. Biochemistry results
D. Immunology results
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